DATA AVAILABILITY STATEMENT : The raw data is available on NCBI’s Sequence Read Archive (SRA)
database under BioProject: PRJNA803487.
SUPPLEMENTARY MATERIALS : FIGURE S1: Soil textures of the
sampling sites; FIGURE S2: Distribution of plant groups across the six study sites; FIGURE S3: Plant species distribution across the study sites. The legume species are represented by asterisk (*) on
the legend. The abbreviations represent: BRIN, Bromus inermis; POPR, Poa pratensis; PHAR, Phalaris
arundinacea; CA, Carex sp.; NA, Nassella sp.; ANGE, Andropogon gerardii; SCSC, Schizachyrium scoparium; BOCU, Bouteloua curtipendula; DIOL, Dichanthelium oligosanthes; CIFL, Cirsium flodmanii;
ASSP, Asclepias speciose; SOMI, Solidago missouriensis; HEMA, Helianthus maximiliani; ANCA, Anemone
canadensis; SOCA, Solidago canadensis; GLLE, Glycyrrhiza lepidota; PH, Physalis sp.; AMCA*, Amorpha canescens; DAPU*, Dalea purpurea; PE*, Pediomelum sp.; RO, Rosa sp.; FIGURE S4: Soil chemical
properties across sampling sites within the sampling time points with Kruskal–Wallis test results;
FIGURE S5: Alpha-diversity of the bacterial communities across 6 months. (a) Shannon diversity and
(b) Pielou Evenness.; FIGURE S6: Taxa differences across at least one time point across the seasons
(p < 0.01). The names of the phyla shown here are based on the taxonomic profile downloaded from
the Greengenes database, however, some of the phylum names have recently been changed [38];
TABLE S1. G-Block solutions; TABLE S2. Primers used for nitrogen cycle genes. SUPPLEMENTARY DATA S3.
R
2 values of the qPCR assays. SUPPLEMENTARY DATA S4. OTU table.