SUPPLEMENTARY FILE 1: FIGURE S1. PCR products were resolved 1% ethidium-bromide stained agarose gel (8V for 1.5 hrs) to check for any contamination. The DNA isolated from whole fly was amplified targeting trypanosomal ITS1 gene. Lane: M 10- bp marker, Bf (reaction buffer), wt (PCR water), TB (T. brucei ILTat 1.4) TV (T. vivax IL 2136), TC (T. congolense savannah (IL3000)), and TE (T. evansi KETRI 2479), F1- F10 DNA sample from H. camelina flies. The absence of PCR product under Bf, and wt show no contamination from extraction buffer.
SUPPLEMENTARY FILE 2: FIGURE S2. Number of H. camelina recaptured at the specified distance from pint of release. Number in parenthesis shows percentage of flies recaptured.
SUPPLEMENTARY FILE 3: FIGURE S3. (A) PCR products were resolved 1% ethidium-bromide stained agarose gel (8V for 1.5 hrs) to check for trypanosomes in blood and lymph node aspirate. The DNA isolated from blood and lymph node aspirate was amplified targeting trypanosomal ITS1 gene. Lane: M 10- bp marker, -Ve (reaction buffer), TE (T. evansi KETRI 2479) TV (T. vivax IL 2136), TC (T. congolense savannah (IL3000)), and LN_C1, LN_C2, DNA sample from two camel lymph node aspirate, B_C1 and B_C2 DNA from corresponding blood samples from the same camel. The result shows both samples of the lymph node aspirate were positive, while blood samples were negative from the same camel. (B) Five camels blood and lymph node aspirate were analysed, only camel five lymph node aspirate was positive for T.vivax but blood sample from the same camel was negative.
SUPPLEMENTARY FILE 4. TABLE S1. Trypanosomes identified based on ITS1 gene sequence from different host included in Fig.6.
DATA AVAILABILITY: All data generated or analysed in this study are included in the article and as additional files. The newly generated sequences were deposited in the NCBI Nucleotide database under the accession numbers listed in Supplementary table.