Characterisation of a proposed Nucleorhabdovirus new to South Africa

Show simple item record Lamprecht, R.L. (Renate Luise) Pietersen, Gerhard Kasdorf, G.G.F. Nel, Louis Hendrik 2009-03-09T10:15:19Z 2009-03-09T10:15:19Z 2009
dc.description.abstract A previously uncharacterised plant rhabdovirus, infecting Bermuda grass (Cynodon dactylon) in the North West Province, South Africa, has been found. To determine the morphology and virion size of this virus, embedded ultra-thin sections of infected plant samples were observed under a transmission electron microscope. The virion distribution within the cell, its bullet-shaped morphology and its size (240 × 63 nm) indicated that this might be a rhabdovirus of the genus Nucleorhabdovirus. Degenerate polymerase chain reaction (PCR) primers were designed by alignment of the polymerase gene sequences of several plant rhabdoviruses in order to identify conserved regions. Standard PCR and sequencing protocols were used to determine a partial polymerase gene sequence of this virus sample which was then compared to the most closely related sequences available on Genbank. The analysis indicated that the virus was indeed most closely related to known nucleorhabdoviruses, with the highest nucleotide sequence similarities being to Maize mosaic virus and Taro vein chlorosis virus (70% and 69.7% respectively). Serological testing indicated that the South African Cynodon rhabdovirus had a close serological relationship with the nucleorhabdovirus Cynodon chlorotic streak virus. en_US
dc.identifier.citation Lamprecht, RL, Pietersen, G, Kasdorf, GGF & Nel, LH 2009, ‘Characterisation of a proposed Nucleorhabdovirus new to South Africa’, European Journal of Plant Pathology, vol. 123, no. 1, pp. 105-110. [] en_US
dc.identifier.issn 1573-8469
dc.identifier.other 10.1007/s10658-008-9339-5
dc.language.iso en en_US
dc.publisher Springer en_US
dc.rights Springer. The original publication is available at en_US
dc.subject Bermuda grass en_US
dc.subject Phylogeny en_US
dc.subject Polymerase gene en_US
dc.subject Rhabdoviridae en_US
dc.subject Serology en_US
dc.subject Taxonomy en_US
dc.title Characterisation of a proposed Nucleorhabdovirus new to South Africa en_US
dc.type Postprint Article en_US

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