SUPPORTING INFORMATION : TABLE S1. Details of specimens included in molecular, morphological (craniodental or
bacular) and/or morphometric analysis of pipistrelloid bats from Angola, Eswatini, South
Africa, Mozambique and Botswana. Abbreviation of Museums: DM (Durban Natural Science
Museum); TM (Ditsong National Museum of Natural History); UNESWA (University of
Eswatini). Availability of skull or baculum specimens indicated by ‘x’. GenBank numbers are
included for specimens having DNA sequences for the three mitochondrial genes,
cytochrome b (cytb), cytochrome oxidase subunit 1 (CO1) and 12S ribosomal RNS (12S).
Specimens marked with asterisks under ‘Skull’ indicate those having skulls available but not
measured in final morphometric analyses.
TABLE S2. Uncorrected P-distances (number of base differences per site from averaging over all sequence pairs) from the CO1 mitochondrial gene are shown, obtained between (bottom left) and within (on diagonals in bold face; ‘n/c’ indicates sample sizes too small for calculation) groups of pipistrelloid bats from Angola, Eswatini and South Africa in relation to GenBank sequences of comparable African species for the CO1 mitochondrial gene. See Supporting Information, Table S1 for details of specimens used in the study. This analysis involved 118 nucleotide sequences. Codon positions included were 1st+2nd+3rd+Noncoding. All ambiguous positions were removed for each sequence pair (pairwise deletion option). There were a total of 478 positions in the final dataset. Analyses were conducted in MEGA X
(Kumar et al., 2018). Abbreviations for groups are as follows: Anan = Afronycteris nana,
Lang = Laephotis angolensis, Lbot = L. botswanae, Lcap(Ang) = L. capensis (Angola),
Lcap(AngSA) = L. capensis (South Africa and Angola), Nanc = Neoromicia anchietae, Nsom
= N. somalica, Nzul = N. zuluensis, Phesp = Pipistrellus hesperidus, Prust(Ang) = P. rusticus
(Angola), Prust(SA) = P. rusticus (southern Africa), Outgroup = outgroup (Miniopterus spp.).
TABLE S3. Uncorrected P-distances (number of base differences per site from averaging over
all sequence pairs) from the 12S RNA mitochondrial gene are shown, obtained between
(bottom left) and within (on diagonals in bold face; ‘n/c’ indicates sample sizes too small for
calculation) groups of pipistrelloid bats from Angola, Eswatini and South Africa in relation to
GenBank sequences of comparable African species for the 12S RNA mitochondrial gene. See
Supporting Information, Table S1 for details of specimens used in the study. This analysis
involved 72 nucleotide sequences. Codon positions included were 1st+2nd+3rd+Noncoding.
All ambiguous positions were removed for each sequence pair (pairwise deletion option).
There were a total of 783 positions in the final dataset. Analyses were conducted in MEGA X
(Kumar et al., 2018). Abbreviations for groups are as follows: Anan(Ang) = Afronycteris
nana (Angola), Anan(EA) = A. nana (East Africa), Anan(WA) = A. nana (West Africa),
Lang = Laephotis angolensis/botswanae (Angola, South Africa, Mozambique), Lcap(EA) =
L. capensis (East Africa), Lcap(SA) = L. capensis (southern Africa), Lnam = L. namibensis,
Nanc = Neoromicia anchietae, Nbem = N. bemainty (Madagascar), Nsom = N. somalica
(East Africa), Nsp_nov = N. sp. nov. (= N. hlandzeni from South Africa and Eswatini),
Nzul(Ang) = N. zuluensis (Angola), Nzul(SA) = N. zuluensis (South Africa, Eswatini),
Phesp(SA) = Pipistrellus hesperidus (South Africa, Eswatini), Pcf.rust(SWA) = P. cf.
rusticus (West Africa, Eswatini), Prust = P. rusticus (Angola), Outgroup = outgroup
(Miniopterus spp).
FIGURE S1. Bayesian phylogeny of mitochondrial cytochrome oxidase one sequences (478
nucleotides) of Vespertilionidae genera Afronycteris, Neoromicia, Laephotis, Pipistrellus
sampled from Angola, South Africa and Eswatini and the outgroup, Miniopterus spp.
(Miniopteridae). Bayesian phylogenetic analysis was performed using the Hasegawa–
Kishino–Yano model incorporating invariant sites and a gamma distribution (HKY+I+G).
Posterior probabilities of > 0.7 are indicated at internal nodes. Samples obtained in this study
are indicated in bold. Branch colours indicate posterior probabilities at each node.
FIGURE S2. Bayesian phylogeny of mitochondrial 12S sequences (478 nucleotides) of
pipistrelle-like Vespertilionidae genera Afronycteris, Neoromicia, Laephotis and Pipistrellus
sampled from Angola, South Africa and Eswatini and outgroup Miniopterus spp.
(Miniopteridae). Bayesian phylogenetic analysis was performed using the Hasegawa–
Kishino–Yano model incorporating invariant sites and a gamma distribution (HKY+I+G).
Posterior probabilities of > 0.7 are indicated at internal nodes. Samples obtained in this study
are indicated in bold. Branch colours indicate posterior probabilities at each node.