JavaScript is disabled for your browser. Some features of this site may not work without it.
Please be advised that the site will be down for maintenance on Sunday, September 1, 2024, from 08:00 to 18:00, and again on Monday, September 2, 2024, from 08:00 to 09:00. We apologize for any inconvenience this may cause.
Novel bio-catalytic degradation of endocrine disrupting compounds in wastewater
Against the backdrop of towering ecological health implications of estrogen
pollution and the inefficacies associated with cost-intensive treatment
techniques, this study recorded the earliest attempt of developing an
inexpensive bacterial laccase-based biocatalysts for biodegradation of EDCs
(Endocrine disrupting compounds), particularly estrogens. First, a central
composite design was used to investigate the interactive effects of
pH (6.0–8.0), inoculum size (100–500 U/mL), and copper (Cu) (25–75 mg/L)
on laccase activity and estrogen degradation respectively. Thereafter,
biocatalysts was synthesized comprising laccase and glass beads or silver
impregnated clay granules (SICG), which was further used to treat estrogen
infused aquatic matrices under different reaction conditions. Maximum laccase
activities and estrogen removal for the two tested laccases were 620 U/mL
(85.8–92.9%) and 689.8 U/mL (86.8–94.6%) for Lysinibacillus sp. BP1 and
Lysinibacillus sp. BP2, respectively, within 72 h, under conditions of optimal
inoculum size and/or Cu concentration. Apart from a higher estrogen removal
rate compared to free laccased, the biocatalysts were more resistant to
temperature, pH and other environmental perturbations, and had enhanced
storage ability and reusability. In comparison to clay, beads had a higher
potential for recyclability and were more stable under certain experimental
factors such as pH, reuse, and temperature, as well as storage conditions.
Immobilized enzymes were able to remove 100% of E2, as well as over 90% of
E1 and EE2, in 24 h, indicating that they could be scaled up to benchtop
bioreactor levels.
Description:
DATA AVAILABILITY STATEMENT : The raw data supporting the conclusions of this article will be
made available by the authors, without undue reservation.