dc.contributor.author |
Lee, Seoung Rak
|
|
dc.contributor.author |
Schalk, Felix
|
|
dc.contributor.author |
Schwitalla, Jan W.
|
|
dc.contributor.author |
Guo, Huijuan
|
|
dc.contributor.author |
Yu, Jae Sik
|
|
dc.contributor.author |
Song, Moonyong
|
|
dc.contributor.author |
Jung, Won Hee
|
|
dc.contributor.author |
De Beer, Z. Wilhelm
|
|
dc.contributor.author |
Beemelmanns, Christine
|
|
dc.contributor.author |
Kim, Ki Hyun
|
|
dc.date.accessioned |
2023-05-10T13:09:46Z |
|
dc.date.available |
2023-05-10T13:09:46Z |
|
dc.date.issued |
2022-06 |
|
dc.description.abstract |
In this study, we analyzed if Actinomadura sp. RB99
produces siderophores that that could be responsible for the
antimicrobial activity observed in co-cultivation studies.
Dereplication of high-resolution tandem mass spectrometry
(HRMS/MS) and global natural product social molecular
networking platform (GNPS) analysis of fungus-bacterium cocultures
resulted in the identification of five madurastatin
derivatives (A1, A2, E1, F, and G1), of which were four new
derivatives. Chemical structures were unambiguously confirmed
by HR-ESI-MS, 1D and 2D NMR experiments, as well as
MS/MS data and their absolute structures were elucidated
based on Marfey’s analysis, DP4+ probability calculation and
total synthesis. Structure analysis revealed that madurastatin
E1 (2) contained a rare 4-imidazolidinone cyclic moiety and
madurastatin A1 (5) was characterized as a Ga3+-complex.
The function of madurastatins as siderophores was evaluated
using the fungal pathogen Cryptococcus neoformans as model
organism. Based on homology models, we identified the
putative NRPS-based gene cluster region of the siderophores
in Actinomadura sp. RB99. |
en_US |
dc.description.department |
Biochemistry |
en_US |
dc.description.department |
Genetics |
en_US |
dc.description.department |
Microbiology and Plant Pathology |
en_US |
dc.description.librarian |
am2023 |
en_US |
dc.description.sponsorship |
The National Research Foundation of Korea, the Postdoctoral Research Program of Sungkyunkwan University, the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation). Open Access funding enabled and organized by Projekt DEAL. |
en_US |
dc.description.uri |
https://www.wiley-vch.de/en/shop/journals/234 |
en_US |
dc.identifier.citation |
Lee, S.R., Schalk, F., Schwitalla, J.W. et al. 2022, 'GNPS - guided discovery of Madurastatin siderophores from the termite-associated actinomadura sp. RB99', Chemistry—A European Journal, vol. 28, no. 36, art. e202200612, pp. 1-9, doi : 10.1002/chem.202200612. |
en_US |
dc.identifier.issn |
0947-6539 (print) |
|
dc.identifier.issn |
1521-3765 (online) |
|
dc.identifier.other |
10.1002/chem.202200612 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/90623 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Wiley |
en_US |
dc.rights |
© 2022 The Authors.
This is an open access article under the terms of the Creative
Commons Attribution Non-Commercial License. |
en_US |
dc.subject |
Metabolomics |
en_US |
dc.subject |
Microbial communication |
en_US |
dc.subject |
Natural products |
en_US |
dc.subject |
Siderophores |
en_US |
dc.subject |
High-resolution tandem mass spectrometry (HRMS/MS) |
en_US |
dc.title |
GNPS - guided discovery of Madurastatin siderophores from the termite-associated actinomadura sp. RB99 |
en_US |
dc.type |
Article |
en_US |