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dc.contributor.author | Lee, Seoung Rak![]() |
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dc.contributor.author | Schalk, Felix![]() |
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dc.contributor.author | Schwitalla, Jan W.![]() |
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dc.contributor.author | Guo, Huijuan![]() |
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dc.contributor.author | Yu, Jae Sik![]() |
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dc.contributor.author | Song, Moonyong![]() |
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dc.contributor.author | Jung, Won Hee![]() |
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dc.contributor.author | De Beer, Z. Wilhelm![]() |
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dc.contributor.author | Beemelmanns, Christine![]() |
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dc.contributor.author | Kim, Ki Hyun![]() |
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dc.date.accessioned | 2023-05-10T13:09:46Z | |
dc.date.available | 2023-05-10T13:09:46Z | |
dc.date.issued | 2022-06 | |
dc.description.abstract | In this study, we analyzed if Actinomadura sp. RB99 produces siderophores that that could be responsible for the antimicrobial activity observed in co-cultivation studies. Dereplication of high-resolution tandem mass spectrometry (HRMS/MS) and global natural product social molecular networking platform (GNPS) analysis of fungus-bacterium cocultures resulted in the identification of five madurastatin derivatives (A1, A2, E1, F, and G1), of which were four new derivatives. Chemical structures were unambiguously confirmed by HR-ESI-MS, 1D and 2D NMR experiments, as well as MS/MS data and their absolute structures were elucidated based on Marfey’s analysis, DP4+ probability calculation and total synthesis. Structure analysis revealed that madurastatin E1 (2) contained a rare 4-imidazolidinone cyclic moiety and madurastatin A1 (5) was characterized as a Ga3+-complex. The function of madurastatins as siderophores was evaluated using the fungal pathogen Cryptococcus neoformans as model organism. Based on homology models, we identified the putative NRPS-based gene cluster region of the siderophores in Actinomadura sp. RB99. | en_US |
dc.description.department | Biochemistry | en_US |
dc.description.department | Genetics | en_US |
dc.description.department | Microbiology and Plant Pathology | en_US |
dc.description.librarian | am2023 | en_US |
dc.description.sponsorship | The National Research Foundation of Korea, the Postdoctoral Research Program of Sungkyunkwan University, the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation). Open Access funding enabled and organized by Projekt DEAL. | en_US |
dc.description.uri | https://www.wiley-vch.de/en/shop/journals/234 | en_US |
dc.identifier.citation | Lee, S.R., Schalk, F., Schwitalla, J.W. et al. 2022, 'GNPS - guided discovery of Madurastatin siderophores from the termite-associated actinomadura sp. RB99', Chemistry—A European Journal, vol. 28, no. 36, art. e202200612, pp. 1-9, doi : 10.1002/chem.202200612. | en_US |
dc.identifier.issn | 0947-6539 (print) | |
dc.identifier.issn | 1521-3765 (online) | |
dc.identifier.other | 10.1002/chem.202200612 | |
dc.identifier.uri | http://hdl.handle.net/2263/90623 | |
dc.language.iso | en | en_US |
dc.publisher | Wiley | en_US |
dc.rights | © 2022 The Authors. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial License. | en_US |
dc.subject | Metabolomics | en_US |
dc.subject | Microbial communication | en_US |
dc.subject | Natural products | en_US |
dc.subject | Siderophores | en_US |
dc.subject | High-resolution tandem mass spectrometry (HRMS/MS) | en_US |
dc.title | GNPS - guided discovery of Madurastatin siderophores from the termite-associated actinomadura sp. RB99 | en_US |
dc.type | Article | en_US |