dc.contributor.author |
Das, Bikram K.
|
|
dc.contributor.author |
Rubel, Robiul Islam
|
|
dc.contributor.author |
Gupta, Surbhi
|
|
dc.contributor.author |
Wu, Yajun
|
|
dc.contributor.author |
Wei, Lin
|
|
dc.contributor.author |
Brözel, Volker Siegfried
|
|
dc.date.accessioned |
2023-03-31T12:24:39Z |
|
dc.date.available |
2023-03-31T12:24:39Z |
|
dc.date.issued |
2022-10-16 |
|
dc.description |
DATA AVAILABILITY STATEMENT : The raw data is available on NCBI’s Sequence Read Archive (SRA) database under BioProject: PRJNA854759. |
en_US |
dc.description |
SUPPLEMENTARY MATERIAL : FIGURE S1: Alpha diversity using Shannon index, Bacterial (A) and Fungal (B) asterisk represent comparisons made using Kruskal–Wallis test with *p < 0.05; FIGURE S2: Bubble plot showing prokaryotic species significantly different for at least one treatment. Multiple dots together signify same grouping across different phyla; TABLE S1: Corn yield and Biomass; TABLE S2: Fertilizer descriptions (wt. % amount with moistures); TABLE S3: Soil Properties; TABLE S4: Commercial fertilizer ingredients; TABLE S5: Taxonomic distribution of the prokaryotic community in soil for each treatment; TABLE S6: Taxa different in high yield compared to other treatments at lowest taxa; TABLE S7: Taxonomic distribution of the fungal community in soil for each treatment; TABLE S8: Genera of fungus significantly different for treatment method. |
en_US |
dc.description.abstract |
Controlled-release Nitrogen Fertilizers (CRNFs) are an effective fertilization technique by
minimizing nutrient loss and making Nitrogen (N) available to plants as they grow. Biochar-based
CRNF (BCRNF) technologies have been demonstrated very promising in increase of corn yield.
Despite the beneficial effects of BCRNFs, their impacts on prokaryotic and fungal soil communities
are not well evaluated. Different formulations of BCRNF were developed to investigate their effects
on corn productivity. We analyzed the soil microbes and their functional potential under different
BCRNF regimes using amplified V3–V4 region of 16s rRNA for determining prokaryotic, and ITS
genes for fungal communities. The soil prokaryotic diversity was similar across the treatments,
with differences in prokaryotic genera with relative abundance of 0.1% or less in the soil (p < 0.05).
In contrast, the fungal community diversity was different only for unfertilized soil. It had a high
relative abundance for Aspergillus. Genus level comparison showed that Pseudofabraea was higher in
Bioasphalt-based BCRNF compared to other treatments. Moreover, the N-fixing communities in soil
were also similar across the treatments. At genus level, Microvirga, Azospirillum, and Methyloprofundus
were highest in no-fertilizer control. The functional potential predictions using PICRUSt2 portrayed
a consistent N-cycling functions across the treatments. However, the predicted gene functions related
to nitrous-oxide reductase (nosZ) and hydroxylamine reductase (hcp) were significantly lower in soil
receiving BCRNF containing biosolid. Overall, BCRNF treatments previously identified to increase
corn yield displayed minimal shifts in the soil microbial communities. Thus, such novel fertilization
would enable increased crop yield without affecting soil communities leading to sustainable crop
production. |
en_US |
dc.description.department |
Biochemistry |
en_US |
dc.description.department |
Genetics |
en_US |
dc.description.department |
Microbiology and Plant Pathology |
en_US |
dc.description.librarian |
am2023 |
en_US |
dc.description.sponsorship |
The South Dakota Governor’s Office of Economic Development and the USDA NIFA through the North Central Regional Sun Grant Center and Hatch Projects of the South Dakota Agricultural Experimental Station. |
en_US |
dc.description.uri |
https://www.mdpi.com/journal/agriculture |
en_US |
dc.identifier.citation |
Das, B.K.; Rubel, R.I.;
Gupta, S.;Wu, Y.;Wei, L.; Brözel, V.S.
Impacts of Biochar-Based
Controlled-Release Nitrogen
Fertilizers on Soil Prokaryotic and
Fungal Communities. Agriculture
2022, 12, 1706. https://DOI.org/10.3390/agriculture12101706. |
en_US |
dc.identifier.issn |
2077-0472 |
|
dc.identifier.other |
10.3390/agriculture12101706 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/90312 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
MDPI |
en_US |
dc.rights |
© 2022 by the authors.
Licensee MDPI, Basel, Switzerland.
This article is an open access article
distributed under the terms and
conditions of the Creative Commons
Attribution (CC BY) license. |
en_US |
dc.subject |
Nitrogen |
en_US |
dc.subject |
Corn |
en_US |
dc.subject |
Soil microbiome |
en_US |
dc.subject |
Microbial functions |
en_US |
dc.subject |
Controlled-release nitrogen fertilizer (CRNF) |
en_US |
dc.subject |
Biochar-based CRNF (BCRNF) |
en_US |
dc.title |
Impacts of biochar-based controlled-release nitrogen fertilizers on soil prokaryotic and fungal communities |
en_US |
dc.type |
Article |
en_US |