dc.contributor.author |
Zhang, Limei
|
|
dc.contributor.author |
Padilla-Flores, Teresa
|
|
dc.contributor.author |
Hernandez, Vito S.
|
|
dc.contributor.author |
Zetter, Mario A.
|
|
dc.contributor.author |
Campos-Lira, Elba
|
|
dc.contributor.author |
Escobar, Laura I.
|
|
dc.contributor.author |
Millar, Robert P.
|
|
dc.contributor.author |
Eiden, Lee E.
|
|
dc.date.accessioned |
2023-02-15T08:00:01Z |
|
dc.date.available |
2023-02-15T08:00:01Z |
|
dc.date.issued |
2022-09 |
|
dc.description.abstract |
Hypothalamic arginine vasopressin (AVP)-containing magnocellular neurosecretory neurons (AVPMNN) emit collaterals to synaptically innervate limbic regions influencing learning, motivational behaviour, and fear responses. Here, we characterize the dynamics of expression changes of two key determinants for synaptic strength, the postsynaptic density (PSD) proteins AMPAR subunit GluA1 and PSD scaffolding protein 95 (PSD95), in response to in vivo manipulations of AVPMNN neuronal activation state, or exposure to exogenous AVP ex vivo. Both long-term water deprivation in vivo, which powerfully upregulates AVPMNN metabolic activity, and exogenous AVP application ex vivo, in brain slices, significantly increased GluA1 and PSD95 expression as measured by western blotting, in brain regions reportedly receiving direct ascending innervations from AVPMNN (i.e., ventral hippocampus, amygdala and lateral habenula). By contrast, the visual cortex, a region not observed to receive AVPMNN projections, showed no such changes. Ex vivo application of V1a and V1b antagonists to ventral hippocampal slices ablated the AVP stimulated increase in postsynaptic protein expression measured by western blotting. Using a modified expansion microscopy technique, we were able to quantitatively assess the significant augmentation of PSD95 and GLUA1 densities in subcellular compartments in locus coeruleus tyrosine hydroxylase immunopositive fibres, adjacent to AVP axon terminals. Our data strongly suggest that the AVPMNN ascending system plays a role in the regulation of the excitability of targeted neuronal circuits through upregulation of key postsynaptic density proteins corresponding to excitatory synapses. |
en_US |
dc.description.department |
Immunology |
en_US |
dc.description.librarian |
hj2023 |
en_US |
dc.description.sponsorship |
Consejo Nacional de Ciencia y Tecnología,
México CONACYT; National Institute of Mental Health;
Universidad Nacional Aut onoma de México. |
en_US |
dc.description.uri |
http://wileyonlinelibrary.com/journal/jne |
en_US |
dc.identifier.citation |
Zhang, L.M., Padilla-Flores, T., Hernandez, V.S., et al. Vasopressin acts as a synapse organizer in limbic regions by boosting PSD95 and GluA1 expression.
Journal of Neuroendocrinology 2022; 34(9): e13164. doi:10.1111/jne.13164. |
en_US |
dc.identifier.issn |
0953-8194 (print) |
|
dc.identifier.issn |
1365-2826 (online) |
|
dc.identifier.other |
10.1111/jne.13164 |
|
dc.identifier.uri |
https://repository.up.ac.za/handle/2263/89542 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Wiley |
en_US |
dc.rights |
© 2022 The Authors. Journal of Neuroendocrinology published by John Wiley & Sons Ltd on behalf of British Society for Neuroendocrinology.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License. |
en_US |
dc.subject |
Central amygdala |
en_US |
dc.subject |
Expansion microscopy |
en_US |
dc.subject |
Arginine vasopressin (AVP) |
en_US |
dc.subject |
Ventral hippocampus |
en_US |
dc.subject |
Water deprivation |
en_US |
dc.subject |
Western blotting |
en_US |
dc.subject |
Magnocellular neurosecretory neurons (MNN) |
en_US |
dc.subject |
Postsynaptic density (PSD) |
en_US |
dc.subject |
PSD scaffolding protein 95 (PSD95) |
en_US |
dc.title |
Vasopressin acts as a synapse organizer in limbic regions by boosting PSD95 and GluA1 expression |
en_US |
dc.type |
Article |
en_US |