Vasopressin acts as a synapse organizer in limbic regions by boosting PSD95 and GluA1 expression

Show simple item record Zhang, Limei Padilla-Flores, Teresa Hernandez, Vito S. Zetter, Mario A. Campos-Lira, Elba Escobar, Laura I. Millar, Robert P. Eiden, Lee E. 2023-02-15T08:00:01Z 2023-02-15T08:00:01Z 2022-09
dc.description.abstract Hypothalamic arginine vasopressin (AVP)-containing magnocellular neurosecretory neurons (AVPMNN) emit collaterals to synaptically innervate limbic regions influencing learning, motivational behaviour, and fear responses. Here, we characterize the dynamics of expression changes of two key determinants for synaptic strength, the postsynaptic density (PSD) proteins AMPAR subunit GluA1 and PSD scaffolding protein 95 (PSD95), in response to in vivo manipulations of AVPMNN neuronal activation state, or exposure to exogenous AVP ex vivo. Both long-term water deprivation in vivo, which powerfully upregulates AVPMNN metabolic activity, and exogenous AVP application ex vivo, in brain slices, significantly increased GluA1 and PSD95 expression as measured by western blotting, in brain regions reportedly receiving direct ascending innervations from AVPMNN (i.e., ventral hippocampus, amygdala and lateral habenula). By contrast, the visual cortex, a region not observed to receive AVPMNN projections, showed no such changes. Ex vivo application of V1a and V1b antagonists to ventral hippocampal slices ablated the AVP stimulated increase in postsynaptic protein expression measured by western blotting. Using a modified expansion microscopy technique, we were able to quantitatively assess the significant augmentation of PSD95 and GLUA1 densities in subcellular compartments in locus coeruleus tyrosine hydroxylase immunopositive fibres, adjacent to AVP axon terminals. Our data strongly suggest that the AVPMNN ascending system plays a role in the regulation of the excitability of targeted neuronal circuits through upregulation of key postsynaptic density proteins corresponding to excitatory synapses. en_US
dc.description.department Immunology en_US
dc.description.librarian hj2023 en_US
dc.description.sponsorship Consejo Nacional de Ciencia y Tecnología, México CONACYT; National Institute of Mental Health; Universidad Nacional Aut onoma de México. en_US
dc.description.uri en_US
dc.identifier.citation Zhang, L.M., Padilla-Flores, T., Hernandez, V.S., et al. Vasopressin acts as a synapse organizer in limbic regions by boosting PSD95 and GluA1 expression. Journal of Neuroendocrinology 2022; 34(9): e13164. doi:10.1111/jne.13164. en_US
dc.identifier.issn 0953-8194 (print)
dc.identifier.issn 1365-2826 (online)
dc.identifier.other 10.1111/jne.13164
dc.language.iso en en_US
dc.publisher Wiley en_US
dc.rights © 2022 The Authors. Journal of Neuroendocrinology published by John Wiley & Sons Ltd on behalf of British Society for Neuroendocrinology. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License. en_US
dc.subject Central amygdala en_US
dc.subject Expansion microscopy en_US
dc.subject Arginine vasopressin (AVP) en_US
dc.subject Ventral hippocampus en_US
dc.subject Water deprivation en_US
dc.subject Western blotting en_US
dc.subject Magnocellular neurosecretory neurons (MNN) en_US
dc.subject Postsynaptic density (PSD) en_US
dc.subject PSD scaffolding protein 95 (PSD95) en_US
dc.title Vasopressin acts as a synapse organizer in limbic regions by boosting PSD95 and GluA1 expression en_US
dc.type Article en_US

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