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dc.contributor.author | Marais, Adriaan Albertyn Scheepers![]() |
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dc.contributor.author | Laurens, Johannes B.![]() |
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dc.date.accessioned | 2009-02-19T07:22:45Z | |
dc.date.available | 2009-02-19T07:22:45Z | |
dc.date.issued | 2008 | |
dc.description.abstract | Amphetamine and related derivatives are widely abused central- and psychostimulants. Detection of certain derivatives, such as methcathinone, by commonly available immunoassay screening techniques is insufficient. Multi-analyte confirmations for amphetamine type stimulants are therefore required, but traditional gas chromatography–mass spectrometry methods necessitate lengthy analytical procedures with prolonged sample turn-around times. A validated rapid GC–MS assay for urinary confirmation of amphetamine, methamphetamine, methcathinone, ephedrine, norephedrine, methylenedioxyamphetamine, methylenedioxymethamphetamine, methylenedioxyethylamphetamine and N-methyl-1-(3,4 methylenedioxyphenyl)-2-butanamine is reported. The method entailed in situ derivatization of urine specimens by extractive acylation with entafluoropropionic anhydride, followed by rapid chromatography on a microbore capillary column. Analytes were separated in less than 3 min and quantified simultaneously by selected-ionmonitoring using stable isotope substituted internal standards. The total instrument cycle-time was 6 min per sample. The limits of detection were between 1.5 ng/mL and 6.25 ng/mL for the various analytes. Intermediate precision and accuracy were in the range of 6.3–13.8% and 90.5–107.3% for the respective analytes at the lower limit of quantitation, and between 5.8–12.6% and 95.4–103.1% for the high control. Long-term storage of methcathinone positive specimens at -20º C proved insufficient stability of this analyte. The proposed assay is precise and accurate for confirmation of amphetamine and derivatives in urine. The complementary approach of extractive-derivatization and fast GC–MS analysis is especially applicable in routine clinical settings where reduced sample turnaround times are required. Further investigation of cathinone as a possible metabolite of methcathinone is warranted, based on results from analyzed authentic urine samples. | en_US |
dc.identifier.citation | Marais, AAS & Laurens, JB 2008,'Rapid GC–MS confirmation of amphetamines in urine by extractive acylation',Forensic Science International, doi:10.1016/j.forsciint.2008.10.021 | en_US |
dc.identifier.isbn | 0379-0738 | |
dc.identifier.other | 10.1016/j.forsciint.2008.10.021 | |
dc.identifier.uri | http://hdl.handle.net/2263/8942 | |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | Elsevier | en_US |
dc.subject | Extractive acylation | en_US |
dc.subject | Methcathinone | en_US |
dc.subject | Amphetamines | en_US |
dc.subject | Rapid GC-MS | en_US |
dc.subject | Isotope dilution | en_US |
dc.subject.lcsh | Amphetamines | |
dc.subject.lcsh | Urine -- Analysis | |
dc.subject.mesh | Monomethylpropion | |
dc.title | Rapid GC–MS confirmation of amphetamines in urine by extractive acylation | en_US |
dc.type | Postprint Article | en_US |