The influence of non-toxic concentrations of DDT and DDE on the old world vulture estrogen receptor alpha

Show simple item record Naidoo, Vinny Katsu, Y. Iguchi, Taisen 2009-02-17T11:04:13Z 2009-02-17T11:04:13Z 2008
dc.description.abstract Seven of the nine vulture species in South Africa are listed as endangered on the International Union for the Conservation of Nature (IUCN) red list. From these, the Cape Griffon vulture (Gyps corprotheres) is the most endangered species in the region. Although inadequate nutritional support has been blamed on the constant decline in populations, the process of vulture restaurants has failed to improve the population status over the last twenty years. One possible reason for the decline may be an underlying reproductive disorder as described in endocrine disruptive syndromes. Both DDT and p,p0-DDE have been detected previously at very high concentrations in the mid 1980s, with lower concentrations still being detectable as late as 2001. To establish the effect of DDT and DDE, the vulture estrogen receptor a (ERa) was sequenced from two species using 50 and 30 rapid amplification cDNA ends (RACE). Using transient transfected mammalian cell assays, vulture ERa estrogen-dependent transcription activity was validated using various estrogens and DDT derivatives. The receptor assay was sensitive to p,p0-DDT, o,p0-DDT and p,p0-DDE with EC50 of 2.41 x 10-6, 3.47 x 10-7 and 3.81 x 10-5 M. When compared to results obtained from human, zebrafish, chicken, salamander and turtle, the vulture ERa showed high sensitivity to o,p0-DDT and intermediately responsive to p,p0-DDE. Vulture ERa is, however, not responsive to the DDT and DDE levels reported in the plasma of vultures from the last population survey, indicating that the Southern African vulture are not currently exposed to disruptive levels of these contaminants. en
dc.identifier.citation Naidoo, V, Katsu, Y, Iguchi, T 2009, 'The influence of non-toxic concentrations of DDT and DDE on the old world Vulture estrogen receptor alpha', General and Comparative Endocrinology, vol.159, no. 2-3,pp. 188-195. [] en
dc.identifier.issn 0016-6480
dc.identifier.other 10.1016/j.ygcen.2008.08.010
dc.identifier.other 8621439700
dc.identifier.other A-1508-2008
dc.language.iso en en
dc.publisher Elsevier en
dc.rights Elsevier en
dc.subject DDT en
dc.subject DDE en
dc.subject Endocrine disruptors en
dc.subject Vultures en
dc.subject Organochlorine en
dc.subject Estrogen receptors en
dc.subject.lcsh Vultures -- South Africa en
dc.subject.lcsh Old World vultures en
dc.subject.lcsh Wildlife conservation en
dc.title The influence of non-toxic concentrations of DDT and DDE on the old world vulture estrogen receptor alpha en
dc.type Postprint Article en

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