Identification of a novel Babesia sp. from a sable antelope (Hippotragus niger Harris, 1838)

Show simple item record Oosthuizen, Marinda C. Zweygarth, Erich Collins, Nicola E. Troskie, Milana Penzhorn, Barend Louis 2009-02-06T07:41:18Z 2009-02-06T07:41:18Z 2008-07
dc.description.abstract Babesiosis in a sable antelope (Hippotragus niger Harris, 1838) was first reported in 1930; the parasite was named Babesia irvinesmithi. Recently, specimens from an adult sable that presented with a sudden onset of disease and that subsequently died during immobilization were submitted for molecular characterization. Microscopic examination of thin blood smears revealed the presence of small piroplasms. DNA was extracted from blood samples; the V4 variable region of the 18S rRNA gene was amplified and analyzed using the reverse line blot (RLB) assay. Amplicons did not hybridize with any of the Babesia or Theileria species-specific probes present on the blot and hybridized only with a Babesia or Theileria genus-specific probe, suggesting the presence of a novel species. The full-length 18S rRNA gene sequence was obtained and aligned with published sequences of related genera, and phylogenetic trees were constructed. Sequence similarity analyses indicated that a Babesia species, designated Babesia sp. (sable), was present. The sequence showed its highest similarity to B. orientalis and to an unnamed Babesia species previously detected in bovine samples. The latter was later established to be Babesia occultans. A Babesia sp. (sable)-specific RLB oligonucleotide probe was designed and used to screen 200 South African sable samples, but so far, no other sample has been found to be positive for the presence of Babesia sp. (sable) DNA. In summary, we identified a novel piroplasm parasite from a sable antelope that died from an unknown illness. While the parasite was observed in blood smears, there is no direct evidence that it was the cause of death. en
dc.identifier.citation Oosthuizen, MC, Zweygarth, E, Collins, NE, Troskie, M & Penzhorn, BL 2008, 'Identification of a novel Babesia sp. from a sable antelope (Hippotragus niger Harris, 1838)', Journal of Clinical Microbiology, vol. 46, no. 7, pp. 2247–2251. [] en
dc.identifier.issn 0095-1137
dc.identifier.other 7004592997
dc.identifier.other 7103250386
dc.identifier.other J-7222-2013
dc.identifier.other 35609781000
dc.identifier.other N-8706-2014
dc.identifier.other O-6342-2014
dc.language.iso en en
dc.publisher American Society for Microbiology en
dc.relation.requires Adobe Acrobat Reader en
dc.rights American Society for Microbiology en
dc.subject Babesia sp. en
dc.subject Hippotragus niger en
dc.subject.lcsh Babesiosis en
dc.subject.lcsh Sable antelope en
dc.title Identification of a novel Babesia sp. from a sable antelope (Hippotragus niger Harris, 1838) en
dc.type Article en

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