dc.contributor.author |
Sandhu, Armaan Kaur
|
|
dc.contributor.author |
Subramanian, Senthil
|
|
dc.contributor.author |
Brözel, Volker Siegfried
|
|
dc.date.accessioned |
2022-09-22T13:17:05Z |
|
dc.date.available |
2022-09-22T13:17:05Z |
|
dc.date.issued |
2021-11-15 |
|
dc.description.abstract |
Soybean roots are colonized and nodulated by multiple strains of compatible nitrogenfixing
rhizobia primarily belonging to the Genus Bradyrhizobium. Motility towards the root and
attachment to root hairs are key determinants of competitive colonization and subsequent nodulation.
Bacterial surface properties and motility are known to vary with chemical composition of the culture
medium, and root adhesion and nodulation occur in a soil environment rather than laboratory
medium. We asked whether the nodulation-promoting factors motility, surface hydrophobicity
and surface adhesion of Bradyrhizobium are affected by growth in a soil nutrient environment.
B. diazoefficiens USDA 110, 126, 3384, and B. elkanii USDA 26 were grown in mineral salt medium with
peptone, yeast extract and arabinose (PSY), and in a soil extracted soluble organic matter (SESOM)
medium. Surface hydrophobicity was determined by partitioning into hydrocarbon, motility by
transition through soft agar, and surface-exposed saccharides by lectin profiling, followed by biofilm
formation and soybean root adhesion capacity of populations. SESOM-grown populations were
generally less motile and more hydrophobic. They bound fewer lectins than PSY-grown populations,
indicating a simpler surface saccharide profile. SESOM populations of USDA 110 did not form
detectable biofilm, but showed increased binding to soy roots. Our results indicate that growth in a
soil environment impacts surface properties, motility, and subsequent soy root adhesion propensity.
Hence, evaluation of Bradyrhizobium for nodulation efficiency should be performed using soil from
the specific field where the soybeans are to be planted, rather than laboratory culture media. |
en_US |
dc.description.department |
Biochemistry |
en_US |
dc.description.department |
Genetics |
en_US |
dc.description.department |
Microbiology and Plant Pathology |
en_US |
dc.description.librarian |
am2022 |
en_US |
dc.description.sponsorship |
The National Science Foundation/EPSCoR RII Track-1: Building on The 2020 Vision: Expanding Research, Education and Innovation in South Dakota and by the South Dakota Board of Regents. |
en_US |
dc.description.uri |
https://www.mdpi.com/journal/nitrogen |
en_US |
dc.identifier.citation |
Sandhu, A.K.;
Subramanian, S.; Brözel, V.S. Surface
Properties and Adherence of
Bradyrhizobium diazoefficiens to Glycine
max Roots Are Altered When Grown
in Soil Extracted Nutrients. Nitrogen
2021, 2, 461–473. https://DOI.org/10.3390/nitrogen2040031. |
en_US |
dc.identifier.issn |
2504-3129 |
|
dc.identifier.other |
10.3390/nitrogen2040031 |
|
dc.identifier.uri |
https://repository.up.ac.za/handle/2263/87303 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
MDPI |
en_US |
dc.rights |
© 2021 by the authors.
Licensee MDPI, Basel, Switzerland.
This article is an open access article
distributed under the terms and
conditions of the Creative Commons
Attribution (CC BY) license. |
en_US |
dc.subject |
Bradyrhizobium |
en_US |
dc.subject |
Attachment |
en_US |
dc.subject |
Root |
en_US |
dc.subject |
Biofilm |
en_US |
dc.subject |
Lectin |
en_US |
dc.subject |
Soybean |
en_US |
dc.subject |
Soil |
en_US |
dc.subject |
Hydrophobicity |
en_US |
dc.title |
Surface properties and adherence of Bradyrhizobium diazoefficiens to Glycine max roots are altered when grown in soil extracted nutrients |
en_US |
dc.type |
Article |
en_US |