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dc.contributor.author | Paweska, Janusz Tadeusz | |
dc.contributor.author | Jansen van Vuren, Petrus | |
dc.contributor.author | Storm, Nadia | |
dc.contributor.author | Markotter, Wanda | |
dc.contributor.author | Kemp, Alan | |
dc.date.accessioned | 2022-04-28T09:55:41Z | |
dc.date.available | 2022-04-28T09:55:41Z | |
dc.date.issued | 2021-11 | |
dc.description.abstract | This study aimed to determine the vector competence of bat-associated nycteribiid flies (Eucamsipoda africana) for Marburg virus (MARV) in the Egyptian Rousette Bat (ERB), Rousettus aegyptiacus. In flies fed on subcutaneously infected ERBs and tested from 3 to 43 days post infection (dpi), MARV was detected only in those that took blood during the peak of viremia, 5–7 dpi. Seroconversion did not occur in control bats in contact with MARV-infected bats infested with bat flies up to 43 days post exposure. In flies inoculated intra-coelomically with MARV and tested on days 0–29 post inoculation, only those assayed on day 0 and day 7 after inoculation were positive by q-RT-PCR, but the virus concentration was consistent with that of the inoculum. Bats remained MARV-seronegative up to 38 days after infestation and exposure to inoculated flies. The first filial generation pupae and flies collected at different times during the experiments were all negative by q-RT-PCR. Of 1693 nycteribiid flies collected from a wild ERB colony in Mahune Cave, South Africa where the enzootic transmission of MARV occurs, only one (0.06%) tested positive for the presence of MARV RNA. Our findings seem to demonstrate that bat flies do not play a significant role in the transmission and enzootic maintenance of MARV. However, ERBs eat nycteribiid flies; thus, the mechanical transmission of the virus through the exposure of damaged mucous membranes and/or skin to flies engorged with contaminated blood cannot be ruled out. | en_US |
dc.description.department | Medical Virology | en_US |
dc.description.librarian | pm2022 | en_US |
dc.description.uri | http://www.mdpi.com/journal/viruses | en_US |
dc.identifier.citation | Pawęska, J.T., Jansen van Vuren, P., Storm, N., Markotter, W. & Kemp, A. Vector Competence of Eucampsipoda africana (Diptera: Nycteribiidae) for Marburg Virus Transmission in Rousettus aegyptiacus (Chiroptera: Pteropodidae). Viruses 2021, 13, 2226. https://doi.org/10.3390/v13112226. | en_US |
dc.identifier.issn | 1999-4915 (online) | |
dc.identifier.other | 10.3390/v13112226 | |
dc.identifier.uri | https://repository.up.ac.za/handle/2263/84935 | |
dc.language.iso | en | en_US |
dc.publisher | MDPI | en_US |
dc.rights | © 2021 by the authors. Licensee: MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | en_US |
dc.subject | Marburg virus | en_US |
dc.subject | Bat flies | en_US |
dc.subject | Vector competence | en_US |
dc.subject | Field infection rate | en_US |
dc.subject | Transmission | en_US |
dc.subject | Egyptian rousette bat | en_US |
dc.subject | Nycteribiid flies (Eucamsipoda africana) | en_US |
dc.subject | Egyptian rousette bat (Rousettus aegyptiacus) | en_US |
dc.title | Vector competence of Eucampsipoda africana (Diptera: Nycteribiidae) for Marburg virus transmission in Rousettus aegyptiacus (Chiroptera: Pteropodidae) | en_US |
dc.type | Article | en_US |