dc.contributor.author |
Zhang, Ying
|
|
dc.contributor.author |
Zhou, Yupei
|
|
dc.contributor.author |
Sun, Wei
|
|
dc.contributor.author |
Zhao, Lili
|
|
dc.contributor.author |
Pavlic-Zupanc, Draginja
|
|
dc.contributor.author |
Crous, Pedro W.
|
|
dc.contributor.author |
Slippers, Bernard
|
|
dc.contributor.author |
Dai, Yucheng
|
|
dc.date.accessioned |
2022-03-03T04:41:28Z |
|
dc.date.available |
2022-03-03T04:41:28Z |
|
dc.date.issued |
2021-11-03 |
|
dc.description |
Supplementary Figure 1 | Botryosphaeria gaubae (W 1992-05937,
holotype). (A,B) Ascomata erumpent through the lower side of the leaf. (C)
Squash showing cylindrical or broadly cylindrical asci in cotton blue. (D) Part of
the peridium. (E) Septate pseudoparaphyses in cotton blue. (F-H) Aseptate,
fusiform to ellipsoid ascospores in cotton blue. Scale bars: (A) = 1 mm,
(B) = 200 mm, (C) = 50 mm, (E) = 20 mm, (D,F-H) = 10 m m. |
en_ZA |
dc.description |
Supplementary Figure 2 | Laestadia apocyni (MICH 14281, isotype). (A)
Ascomata erumpent through a piece of twig epidermis. (B) Released, hyaline,
1-septate ascospores. (C) Ascus in water. (D) Line drawing of ascus in water.
Scale bars: (A) = 200 mm, (B-D) = 20 m m. |
en_ZA |
dc.description |
Supplementary Figure 3 | Sphaeria smilacinina (NYS f2818, holotype). (A)
Ascomata erumpent through the twig epidermis. (B,C) Immature asci. (D)
Released ascospores. (E) Line drawing of broadly clavate ascus. Scale bars:
(A) = 500 mm, (B-D) = 20 mm, (E) = 40 m m. |
en_ZA |
dc.description |
Supplementary Figure 4 | One of the most parsimonious trees obtained from
combined ITS, LSU, tub2, and tef1-a sequence data of Botryosphaeria spp.
Outgroup taxa are Neofusicoccum luteum and Neofusicoccum parvum. Maximum
parsimony (MP) support values above 70% and Bayesian posterior probabilities
(PP) support above 80% are shown with MP bootstrap followed by Bayesian PP
(MP/PP) values at the nodes. The species characterized in this study
are in boldface. |
en_ZA |
dc.description |
Supplementary Figure 5 | One of the most parsimonious trees obtained based
on combined ITS, tef1-a, and tub2 sequence data of Neofusicoccum spp.
Outgroup taxon are Botryosphaeria dothidea and B. corticis. Maximum parsimony
(MP) support values above 60% and Bayesian posterior probabilities (PP) support
above 80% are shown with MP/PP, values at the nodes. The species
characterized in this study are in boldface. |
en_ZA |
dc.description |
Supplementary Figure 6 | One of the most parsimonious trees obtained from
LSU sequence dataset of Neofusicoccum spp. Outgroup taxa are Botryosphaeria
corticis and B. dothidea. Maximum parsimony (MP) support values above 70%
and Bayesian posterior probabilities (PP) support above 80% are shown with MP
bootstrap followed by Bayesian PP (MP/PP) values at the nodes. The species
characterized in this study are in boldface. |
en_ZA |
dc.description |
Supplementary Figure 7 | One of the most parsimonious trees obtained from
ITS and LSU sequence dataset of Nothophoma spp. Outgroup taxa is Didymella
calidophila. Maximum likelihood (ML) support values above 50%, Maximum
parsimony (MP) support values above 50%, and Bayesian posterior probabilities
(PP) support above 95% are shown with ML and MP bootstrap followed by
Bayesian PP (MP/PP/ML) values at the nodes. The species characterized in this
study are in boldface. |
en_ZA |
dc.description |
Supplementary Table 1 | Species, specimens and GenBank accession numbers
of sequences used in this study (newly generated sequences are
indicated in bold). |
en_ZA |
dc.description.abstract |
The genus Botryosphaeria includes more than 200 epithets, but only the type
species, Botryosphaeria dothidea and a dozen or more other species have been
identified based on DNA sequence data. The taxonomic status of the other species
remains unconfirmed because they lack either morphological information or DNA
sequence data. In this study, types or authentic specimens of 16 “Botryosphaeria”
species are reassessed to clarify their identity and phylogenetic position. nuDNA
sequences of four regions, ITS, LSU, tef1-a and tub2, are analyzed and considered
in combination with morphological characteristics. Based on the multigene phylogeny
and morphological characters, Botryosphaeria cruenta and Botryosphaeria hamamelidis
are transferred to Neofusicoccum. The generic status of Botryosphaeria aterrima and
Botryosphaeria mirabile is confirmed in Botryosphaeria. Botryosphaeria berengeriana
var. weigeliae and B. berengeriana var. acerina are treated synonyms of B. dothidea.
Botryosphaeria mucosa is transferred to Neodeightonia as Neodeightonia mucosa, and
Botryosphaeria ferruginea to Nothophoma as Nothophoma ferruginea. Botryosphaeria
foliicola is reduced to synonymy with Phyllachorella micheliae. Botryosphaeria abuensis,
Botryosphaeria aesculi, Botryosphaeria dasylirii, and Botryosphaeria wisteriae are
tentatively kept in Botryosphaeria sensu stricto until further phylogenetic analysis is
carried out on verified specimens. The ordinal status of Botryosphaeria apocyni,
Botryosphaeria gaubae, and Botryosphaeria smilacinina cannot be determined, and
tentatively accommodate these species in Dothideomycetes incertae sedis. The
study demonstrates the significance of a polyphasic approach in characterizing type
specimens, including the importance of using of DNA sequence data. |
en_ZA |
dc.description.department |
Forestry and Agricultural Biotechnology Institute (FABI) |
en_ZA |
dc.description.department |
Genetics |
en_ZA |
dc.description.department |
Microbiology and Plant Pathology |
en_ZA |
dc.description.librarian |
am2022 |
en_ZA |
dc.description.sponsorship |
The National Natural Science Foundation of China and NSFC Projects of International Cooperation and Exchanges. |
en_ZA |
dc.description.uri |
http://www.frontiersin.org/Microbiology |
en_ZA |
dc.identifier.citation |
Zhang, Y., Zhou, Y.P., Sun, W., Zhao, L.L., Pavlic-Zupanc, D., Crous, P.W., Slippers, B. & Dai, Y.C. (2021) Toward
a Natural Classification
of Botryosphaeriaceae: A Study of the
Type Specimens of Botryosphaeria
sensu lato.
Frontiers in Microbiology 12:737541.
DOI: 10.3389/fmicb.2021.737541 |
en_ZA |
dc.identifier.issn |
1664-302X (online) |
|
dc.identifier.other |
10.3389/fmicb.2021.737541 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/84307 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Frontiers Media |
en_ZA |
dc.rights |
© 2021 Zhang, Zhou, Sun, Zhao, Pavlic-Zupanc, Crous, Slippers and Dai.
This is an open-access article distributed under the terms of the Creative Commons
Attribution License (CC BY). |
en_ZA |
dc.subject |
Botryosphaeriales |
en_ZA |
dc.subject |
Phylogeny |
en_ZA |
dc.subject |
Sexual stage |
en_ZA |
dc.subject |
Taxonomy |
en_ZA |
dc.subject |
Type specimens |
en_ZA |
dc.title |
Toward a natural classification of Botryosphaeriaceae : a study of the type specimens of Botryosphaeria sensu lato |
en_ZA |
dc.type |
Article |
en_ZA |