Role of the kdpDE regulatory operon of Mycobacterium tuberculosis in modulating bacterial growth in vitro

Show simple item record Cholo, Moloko C. Matjokotja, Maborwa T. Osman, Ayman Gassim Elamin Anderson, Ronald 2022-02-25T11:03:29Z 2022-02-25T11:03:29Z 2021-07-29
dc.description.abstract Bacteria use K+-uptake transporters differentially for adaptation in varying growth conditions. In Mycobacterium tuberculosis, two K+-uptake systems, the Trk comprising the CeoB and CeoC proteins and the Kdp consisting of the two-component system (TCS), KdpDE and KdpFABC, have been characterized, but their selective utilization during bacterial growth has not been completely explored. In the current study, the roles of the M. tuberculosis KdpDE regulatory system alone and in association with the Trk transporters in bacterial growth were investigated by evaluating the growth of M. tuberculosis KdpDE-deletion and KdpDE/Trk (KT)-double knockout mutant strains in planktonic culture under standard growth conditions. The KT-double knockout mutant strain was first constructed using homologous recombination procedures and was evaluated together with the KdpDE-deletion mutant and the wild-type (WT) strains with respect to their rates of growth, K+-uptake efficiencies, and K+-transporter gene expression during planktonic growth. During growth at optimal K+ concentrations and pH levels, selective deletion of the TCS KdpDE (KdpDE-deletion mutant) led to attenuation of bacterial growth and an increase in bacterial K+-uptake efficiency, as well as dysregulated expression of the kdpFABC and trk genes. Deletion of both the KdpDE and the Trk systems (KT-double knockout) also led to severely attenuated bacterial growth, as well as an increase in bacterial K+-uptake efficiency. These results demonstrate that the KdpDE regulatory system plays a key role during bacterial growth by regulating K+ uptake via modulation of the expression and activities of both the KdpFABC and Trk systems and is important for bacterial growth possibly by preventing cytoplasmic K+ overload. en_ZA
dc.description.department Immunology en_ZA
dc.description.librarian am2022 en_ZA
dc.description.sponsorship The South African National Research Foundation en_ZA
dc.description.uri en_ZA
dc.identifier.citation Cholo, M.C., Matjokotja, M.T., Osman, A.G. & Anderson, R. (2021) Role of the kdpDE Regulatory Operon of Mycobacterium tuberculosis in Modulating Bacterial Growth in vitro. Frontiers in Genetics 12:698875. DOI: 10.3389/fgene.2021.698875. en_ZA
dc.identifier.issn 1664-8021 (online)
dc.identifier.other 10.3389/fgene.2021.698875
dc.language.iso en en_ZA
dc.publisher MDPI en_ZA
dc.rights © 2021 Cholo, Matjokotja, Osman and Anderson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). en_ZA
dc.subject Two-component system KdpDE en_ZA
dc.subject KdpFABC system en_ZA
dc.subject Trk system en_ZA
dc.subject K+-uptake systems en_ZA
dc.subject K+ concentration en_ZA
dc.subject pH Level en_ZA
dc.subject Gene expression en_ZA
dc.subject Mycobacterium tuberculosis (MTB) en_ZA
dc.title Role of the kdpDE regulatory operon of Mycobacterium tuberculosis in modulating bacterial growth in vitro en_ZA
dc.type Article en_ZA

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