Inflammation is an important component of the immune response to pathogens and damaged cells. This study aims to assess and compare of anti-inflammatory activities in-vitro and in-silico of essential oils from galls formed on leaves of Pistacia atlantica Desf from two regions in Algeria (four samples from Ain-oussera and four samples from Laghouat). The anti-inflammatory activity was achieved by Nitric oxide inhibitory activity and viability of LPS‑activated RAW 264.7 macrophages, and inhibition activity of 15-Lipoxygenase (LOX), quercetin was used as a positive control. The essential oils from leave gall of Pistacia atlantica species is able to inhibited 15-LOX enzyme with IC50 0.004mg/mL and also inhibited the production of NO produced by macrophages (99.17%) without significant cytotoxic effect (78% cell viability). The molecular docking study confirm the inhibitory effect of the major compounds of the studied essential oil, even ADMET analysis indicate that the major compounds are not toxic.