dc.contributor.author |
Kwofie, Luyanda Laura Illicia
|
|
dc.contributor.author |
Anderson, Ronald
|
|
dc.contributor.author |
Steel, Helen C.
|
|
dc.contributor.author |
Meyer, Pieter Willem Adriaan
|
|
dc.date.accessioned |
2021-12-14T08:12:31Z |
|
dc.date.available |
2021-12-14T08:12:31Z |
|
dc.date.issued |
2021-06 |
|
dc.description.abstract |
BACKGROUND : Anti-human leukocyte antigen antibodies (anti-HLA) play a crucial role in graft. Detection of anti-HLA, both
pre- and post-transplant is a crucial investigation in clinical organ transplantation.
OBJECTIVES : Three methodologies for the detection of lymphocytotoxic antibodies were compared to establish which of
these is best suited to optimise pre-transplant donor-recipient matching.
METHODS : Serum samples from 15 renal transplant patients were tested for the presence of anti-HLA by i) cytotoxic-dependent
cross-match (CDCXM), ii) flow cytometric cross-match (FCXM) and iii) Luminex-based donor specific antibody
cross-match (DSAXM) method, Confirmatory tests for the presence of preformed HLA antibodies were tested using Luminex
methodology.
RESULTS : Two (13%) of the 15 patients had positive HLA Class I antibodies (Ab) using all 3 methods. An additional 2 HLA
Class I Ab were identified with FCXM/CDCXM. DSAXM identified 1 HLA Class I positive, not indicated by CDCXM/
FCXM.
High HLA Class II positivity (40%), identified by CDCXM, while DSAXM and FCXM identified two and one patients,
respectively. CDCXM produced 4 false-positive results confirmed by lymphocyte single antigen (LSA) assay.
CONCLUSIONS : The DSAXM method appears to add value in pre-transplantation screening to identify pre-sensitised patients
that may not reject the donor graft due to the absence of donor-specific antibodies. |
en_ZA |
dc.description.department |
Immunology |
en_ZA |
dc.description.librarian |
am2021 |
en_ZA |
dc.description.uri |
http://www.bioline.org.br/hs |
en_ZA |
dc.identifier.citation |
Kwofie, L., Anderson, R., Steel, H., Meyer, P.W.A. Evaluation of three different laboratory methods to detect preformed human
leukocyte antigen antibodies in a South African kidney transplant population. African Health Sciences 2021;21(2). 735-742. https://dx.DOI.org/ 10.4314/ahs.v21i2.32 |
en_ZA |
dc.identifier.issn |
1680-6905 (print) |
|
dc.identifier.issn |
1729-0503 (online) |
|
dc.identifier.other |
10.4314/ahs.v21i2.32 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/83049 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Makerere University Medical School |
en_ZA |
dc.rights |
© 2021 Kwofie L et al. Licensee African Health Sciences. This is an Open Access article distributed under the terms of the Creative commons Attribution License. |
en_ZA |
dc.subject |
Preformed human leukocyte |
en_ZA |
dc.subject |
Antigen antibodies |
en_ZA |
dc.subject |
Kidney transplant |
en_ZA |
dc.subject |
Population |
en_ZA |
dc.subject |
South Africa (SA) |
en_ZA |
dc.subject |
Anti-human leukocyte antigen antibodies (anti-HLA) |
en_ZA |
dc.title |
Evaluation of three different laboratory methods to detect preformed human leukocyte antigen antibodies in a South African kidney transplant population |
en_ZA |
dc.type |
Article |
en_ZA |