Please be advised that the site will be down for maintenance on Sunday, September 1, 2024, from 08:00 to 18:00, and again on Monday, September 2, 2024, from 08:00 to 09:00. We apologize for any inconvenience this may cause.
dc.contributor.author | Kwofie, Luyanda Laura Illicia![]() |
|
dc.contributor.author | Anderson, Ronald![]() |
|
dc.contributor.author | Steel, Helen C.![]() |
|
dc.contributor.author | Meyer, Pieter Willem Adriaan![]() |
|
dc.date.accessioned | 2021-12-14T08:12:31Z | |
dc.date.available | 2021-12-14T08:12:31Z | |
dc.date.issued | 2021-06 | |
dc.description.abstract | BACKGROUND : Anti-human leukocyte antigen antibodies (anti-HLA) play a crucial role in graft. Detection of anti-HLA, both pre- and post-transplant is a crucial investigation in clinical organ transplantation. OBJECTIVES : Three methodologies for the detection of lymphocytotoxic antibodies were compared to establish which of these is best suited to optimise pre-transplant donor-recipient matching. METHODS : Serum samples from 15 renal transplant patients were tested for the presence of anti-HLA by i) cytotoxic-dependent cross-match (CDCXM), ii) flow cytometric cross-match (FCXM) and iii) Luminex-based donor specific antibody cross-match (DSAXM) method, Confirmatory tests for the presence of preformed HLA antibodies were tested using Luminex methodology. RESULTS : Two (13%) of the 15 patients had positive HLA Class I antibodies (Ab) using all 3 methods. An additional 2 HLA Class I Ab were identified with FCXM/CDCXM. DSAXM identified 1 HLA Class I positive, not indicated by CDCXM/ FCXM. High HLA Class II positivity (40%), identified by CDCXM, while DSAXM and FCXM identified two and one patients, respectively. CDCXM produced 4 false-positive results confirmed by lymphocyte single antigen (LSA) assay. CONCLUSIONS : The DSAXM method appears to add value in pre-transplantation screening to identify pre-sensitised patients that may not reject the donor graft due to the absence of donor-specific antibodies. | en_ZA |
dc.description.department | Immunology | en_ZA |
dc.description.librarian | am2021 | en_ZA |
dc.description.uri | http://www.bioline.org.br/hs | en_ZA |
dc.identifier.citation | Kwofie, L., Anderson, R., Steel, H., Meyer, P.W.A. Evaluation of three different laboratory methods to detect preformed human leukocyte antigen antibodies in a South African kidney transplant population. African Health Sciences 2021;21(2). 735-742. https://dx.DOI.org/ 10.4314/ahs.v21i2.32 | en_ZA |
dc.identifier.issn | 1680-6905 (print) | |
dc.identifier.issn | 1729-0503 (online) | |
dc.identifier.other | 10.4314/ahs.v21i2.32 | |
dc.identifier.uri | http://hdl.handle.net/2263/83049 | |
dc.language.iso | en | en_ZA |
dc.publisher | Makerere University Medical School | en_ZA |
dc.rights | © 2021 Kwofie L et al. Licensee African Health Sciences. This is an Open Access article distributed under the terms of the Creative commons Attribution License. | en_ZA |
dc.subject | Preformed human leukocyte | en_ZA |
dc.subject | Antigen antibodies | en_ZA |
dc.subject | Kidney transplant | en_ZA |
dc.subject | Population | en_ZA |
dc.subject | South Africa (SA) | en_ZA |
dc.subject | Anti-human leukocyte antigen antibodies (anti-HLA) | en_ZA |
dc.title | Evaluation of three different laboratory methods to detect preformed human leukocyte antigen antibodies in a South African kidney transplant population | en_ZA |
dc.type | Article | en_ZA |