Comparison of transcriptional responses and metabolic alterations in three multidrug-resistant model microorganisms, staphylococcus aureus ATCC BAA-39, escherichia coli ATCC BAA196, and acinetobacter baumannii ATCC BAA-1790, on exposure to iodine-containing nano-micelle drug FS-1

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dc.contributor.author Korotetskiy, Ilya S.
dc.contributor.author Shilov, Sergey V.
dc.contributor.author Kuznetsova, Tatyana V.
dc.contributor.author Ilin, Aleksandr I.
dc.contributor.author Joubert, Monique
dc.contributor.author Taukobong, Setshaba
dc.contributor.author Reva, Oleg N.
dc.date.accessioned 2021-12-01T09:46:17Z
dc.date.available 2021-12-01T09:46:17Z
dc.date.issued 2021-03
dc.description.abstract Iodine is one of the oldest antimicrobial agents. Until now, there have been no reports on acquiring resistance to iodine. Recent studies showed promising results on application of iodine-containing nano-micelles, FS-1, against antibiotic-resistant pathogens as a supplement to antibiotic therapy. The mechanisms of the action, however, remain unclear. The aim of this study was to perform a holistic analysis and comparison of gene regulation in three phylogenetically distant multidrug-resistant reference strains representing pathogens associated with nosocomial infections from the ATCC culture collection: Escherichia coli BAA-196, Staphylococcus aureus BAA-39, and Acinetobacter baumannii BAA-1790. These cultures were treated by a 5-min exposure to sublethal concentrations of the iodine-containing drug FS-1 applied in the late lagging phase and the middle of the logarithmic growth phase. Complete genome sequences of these strains were obtained in the previous studies. Gene regulation was studied by total RNA extraction and Ion Torrent sequencing followed by mapping the RNA reads against the reference genome sequences and statistical processing of read counts using the DESeq2 algorithm. It was found that the treatment of bacteria with FS-1 profoundly affected the expression of many genes involved in the central metabolic pathways; however, alterations of the gene expression profiles were species specific and depended on the growth phase. Disruption of respiratory electron transfer membrane complexes, increased penetrability of bacterial cell walls, and osmotic and oxidative stresses leading to DNA damage were the major factors influencing the treated bacteria. IMPORTANCE: Infections caused by antibiotic-resistant bacteria threaten public health worldwide. Combinatorial therapy in which antibiotics are administered together with supplementary drugs improving susceptibility of pathogens to the regular antibiotics is considered a promising way to overcome this problem. An induction of antibiotic resistance reversion by the iodine-containing nano-micelle drug FS-1 has been reported recently. This drug is currently under clinical trials in Kazakhstan against multidrug-resistant tuberculosis. The effects of released iodine on metabolic and regulatory processes in bacterial cells remain unexplored. The current work provides an insight into gene regulation in the antibiotic-resistant nosocomial reference strains treated with iodine-containing nanoparticles. This study sheds light on unexplored bioactivities of iodine and the mechanisms of its antibacterial effect when applied in sublethal concentrations. This knowledge will aid in the future design of new drugs against antibiotic-resistant infections. en_ZA
dc.description.department Biochemistry en_ZA
dc.description.department Genetics en_ZA
dc.description.department Microbiology and Plant Pathology en_ZA
dc.description.librarian pm2021 en_ZA
dc.description.sponsorship The Ministry of Industry and Infrastructural Development of the Republic of Kazakhstan and the National Research Foundation (NRF) of South Africa. en_ZA
dc.description.uri https://journals.asm.org/journal/msystems en_ZA
dc.identifier.citation Korotetskiy IS, Shilov SV, Kuznetsova TV, Ilin AI, Joubert M, Taukobong S, Reva ON. 2021. Comparison of transcriptional responses and metabolic alterations in three multidrugresistant model microorganisms, Staphylococcus aureus ATCC BAA-39, Escherichia coli ATCC BAA-196, and Acinetobacter baumannii ATCC BAA-1790, on exposure to iodine-containing nano-micelle drug FS-1. mSystems 6:e01293-20. https://doi.org/10.1128/mSystems.01293-20. en_ZA
dc.identifier.issn 2379-5077 (online)
dc.identifier.other 10.1128/mSystems.01293-20
dc.identifier.uri http://hdl.handle.net/2263/82926
dc.language.iso en en_ZA
dc.publisher American Society for Microbiology en_ZA
dc.rights © 2021 Korotetskiy et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. en_ZA
dc.subject Transcriptomics en_ZA
dc.subject Escherichia coli en_ZA
dc.subject Staphylococcus aureus en_ZA
dc.subject Acinetobacter baumannii en_ZA
dc.subject Antibiotic resistance en_ZA
dc.subject Iodine en_ZA
dc.subject Nano-micelle en_ZA
dc.title Comparison of transcriptional responses and metabolic alterations in three multidrug-resistant model microorganisms, staphylococcus aureus ATCC BAA-39, escherichia coli ATCC BAA196, and acinetobacter baumannii ATCC BAA-1790, on exposure to iodine-containing nano-micelle drug FS-1 en_ZA
dc.type Article en_ZA


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