Supplementary Movie 1. Scratch assay for GEβ1, GEβ3, and GEβ1/β3 cells. The indicated cell lines were grown to confluency, serum-starved overnight, and scratched with a pipette tip. Scratch closure was stimulated with 10% FCS, and cells were imaged by time-lapse video microscopy. Image intervals; 10 min, imaging time 16 h.
Supplementary Movie 2. Single-cell migration assay for GEβ1, GEβ3, and GEβ1/β3 cells on FN. The indicated cell lines were sparsely seeded on FN and monitored by time-lapse video microscopy. Image intervals; 10 min, imaging time 15 h.
Supplementary Movie 3. Scratch assay for cells expressing β1 or β3 mutants. The indicated cell lines were grown to confluency, serum-starved overnight, and scratched with a pipette tip. Scratch closure was stimulated with 10% FCS, cells were imaged by time-lapse video microscopy. Image intervals; 10 min, imaging time 15 h.
Supplementary Movie 4. Single-cell migration assay for cells expressing β1 or β3 mutants on FN. The indicated cell lines were sparsely seeded on FN (5 μg/ml) and monitored by time-lapse video microscopy. Image intervals; 10 min, imaging time 15 h.
Supplementary Movie 5. Single-cell migration assay for cells expressing β1 together with β3 mutants on FN. The indicated cell lines were sparsely seeded on FN (5 μg/ml) and monitored by time-lapse video microscopy. Image intervals; 10 min, imaging time 15 h.