Macrophage activation assays to evaluate the immunostimulatory capacity of avibacterium paragallinarum in a multivalent poultry vaccine

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dc.contributor.author Van den Biggelaar, Robin H.G.A.
dc.contributor.author Van Eden, Willem
dc.contributor.author Rutten, Victor P.M.G.
dc.contributor.author Jansen, Christine A.
dc.date.accessioned 2021-10-06T05:04:54Z
dc.date.available 2021-10-06T05:04:54Z
dc.date.issued 2020-11-10
dc.description Figure S1: Cytotoxicity of the octavalent vaccine, extracted antigens and purified Av. paragallinarum bacteria, Figure S2: Gram staining of extracted antigens from the tri- and octavalent vaccines, Figure S3: The octavalent vaccine-induced gene expression of IL-10, whereas a trivalent vaccine without Av. paragallinarum antigens did not. en_ZA
dc.description.abstract High-quality vaccines are crucial to prevent infectious disease outbreaks in the poultry industry. In vivo vaccination tests are routinely used to test poultry vaccines for their potency, i.e., their capacity to induce protection against the targeted diseases. A better understanding of how poultry vaccines activate immune cells will facilitate the replacement of in vivo potency tests for in vitro assays. Using the chicken macrophage-like HD11 cell line as a model to evaluate innate immune responses, the current explorative study addresses the immunostimulatory capacity of an inactivated multivalent vaccine for infectious bronchitis, Newcastle disease, egg-drop syndrome, and infectious coryza. The vaccine stimulated HD11 cells to produce nitric oxide and to express pro-inflammatory cytokines IL-1 , TNF, and IL-12p40, chemokines CXCLi1 and CXCLi2, and the anti-inflammatory cytokine IL-10, but only when inactivated Avibacterium paragallinarum, the causative agent of infectious coryza, was present. Lipopolysaccharides from Avibacterium paragallinarum were crucial for the production of nitric oxide and expression of IL-1 and CXCLi1. The described immune parameters demonstrate the capacity of this multivalent vaccine to activate innate immune cells and may in the future, combined with antigen quantification methods, contribute to vaccine quality testing in vitro, hence the replacement of current in vivo vaccination tests. en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.librarian am2021 en_ZA
dc.description.sponsorship The Innovative Medicines Initiative 2 Joint Undertaking en_ZA
dc.description.uri http://www.mdpi.com/journal/vaccines en_ZA
dc.identifier.citation Van den Biggelaar, RHGA, Van Eden, W & Rutten, VPMG 2020, 'Macrophage activation assays to evaluate the immunostimulatory capacity of avibacterium paragallinarum in a multivalent poultry vaccine', Vaccines, vol. 8, no. 4, art. 671, pp. 1-16. en_ZA
dc.identifier.issn 2076-393X (online)
dc.identifier.other 10.3390/vaccines8040671
dc.identifier.uri http://hdl.handle.net/2263/82040
dc.language.iso en en_ZA
dc.publisher MDPI en_ZA
dc.rights © 2020 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license en_ZA
dc.subject Inactivated poultry vaccine en_ZA
dc.subject Avibacterium paragallinarum en_ZA
dc.subject Infectious coryza en_ZA
dc.subject Potency test en_ZA
dc.subject In vitro en_ZA
dc.subject HD11 cell line en_ZA
dc.subject Nitric oxide en_ZA
dc.subject Cytokines en_ZA
dc.subject Lipopolysaccharides en_ZA
dc.title Macrophage activation assays to evaluate the immunostimulatory capacity of avibacterium paragallinarum in a multivalent poultry vaccine en_ZA
dc.type Article en_ZA


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