Biology of a putative male aggregation-sex pheromone in Sirex noctilio (Hymenoptera: Siricidae)

Show simple item record Guignard, Quentin Bouwer, Marc Clement Slippers, Bernard Allison, Jeremy D. 2021-07-27T10:06:21Z 2021-07-27T10:06:21Z 2020-12-31
dc.description S1 Fig. Three dimensional chromatogram showing the two co-eluting peaks (A and B) eluting in the tail of the large major chromatographic peak released by males. The best tentative match for peak A was (Z)-3-decenol and peak B was (Z)-4-decenol in the NIST library. en_ZA
dc.description S2 Fig. Antennal recovery (in %) 30, 45 and 60 sec after 10 000 ng of (Z)-3-decenol puffed (n = 3 males and 3 females). Different letters indicate significant differences of the recovery time. en_ZA
dc.description.abstract A putative male-produced pheromone has recently been described for the global pest of pines, Sirex noctilio, but field-activity has not been demonstrated. This study aimed to investigate the pheromone biology of S. noctilio in more detail. Specifically, we i) analysed effluvia and extracts for additional compounds by gas chromatography coupled with electro-antennographic detection (GC-EAD), mass spectrometry (GC-MS) and two dimensional time of flight mass spectrometry (GC X GC TOF MS), ii) conducted dose-response experiments for putative pheromone components, iii) determined the site of synthesis/ storage of the putative pheromone and iv) determined the release rate of the putative pheromone from males and three types of lures. A blend of four compounds was identified, including the previously described (Z)-3-decenol and (Z)-4-decenol, and two new compounds (Z)-3-octenol and (Z)- 3-dodecenol. All compounds elicited a response from both male and female antennae, but the strength of the response varied according to sex, compound and dose tested. (Z)-3- Decenol and (Z)-3-octenol at lower and higher doses, respectively, elicited larger responses in males and females than the other two compounds. (Z)-3-Octenol and (Z)-4-decenol generally elicited larger female than male antennal responses. The site of synthesis and/or storage in males was determined to be the hind legs, likely in the leg-tendon gland. The relative release rate of the major compound by male wasps was shown to be 90 ± 12.4 ng/min, which is between 4 and 15 times greater than that observed from typical lures used previously. These observations are consistent with the hypothesis that these compounds may mediate lek formation in S. noctilio males and lek location in females. en_ZA
dc.description.department Biochemistry en_ZA
dc.description.department Chemistry en_ZA
dc.description.department Forestry and Agricultural Biotechnology Institute (FABI) en_ZA
dc.description.department Genetics en_ZA
dc.description.department Microbiology and Plant Pathology en_ZA
dc.description.department Zoology and Entomology en_ZA
dc.description.librarian am2021 en_ZA
dc.description.sponsorship The United State Department of Agriculture-Forest Service Forest Health Protection (USDA-FS FHP), National Resources Canada (NRCan), the Tree Protection Cooperative Program (TPCP) and the DSI NRF Center of Excellence in Plant Health Biotechnology (CPHB) in South Africa. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Guignard Q, Bouwer M, Slippers B, Allison J (2020) Biology of a putative male aggregation-sex pheromone in Sirex noctilio (Hymenoptera: Siricidae). PLoS ONE 15(12): e0244943. en_ZA
dc.identifier.issn 1932-6203 (online)
dc.identifier.other 10.1371/journal. pone.0244943
dc.language.iso en en_ZA
dc.publisher Public Library of Science en_ZA
dc.rights © 2020 Guignard et al. This is an open access article distributed under the terms of the Creative Commons Attribution License. en_ZA
dc.subject Sirex noctilio en_ZA
dc.subject Antennae en_ZA
dc.subject Compounds en_ZA
dc.subject Pheromone biology en_ZA
dc.subject Gas chromatography coupled with electro-antennographic detection (GC-EAD) en_ZA
dc.subject Gas chromatography coupled with mass spectrometry (GC-MS) en_ZA
dc.subject Two dimensional time of flight mass spectrometry (GC X GC TOF MS) en_ZA
dc.title Biology of a putative male aggregation-sex pheromone in Sirex noctilio (Hymenoptera: Siricidae) en_ZA
dc.type Article en_ZA

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