Genomic and transcriptomic analysis unveils population evolution and development of pesticide resistance in fall armyworm Spodoptera frugiperda
Gui, Furong; Lan, Tianming; Zhao, Yue; Guo, Wei; Dong, Yang; Fang, Dongming; Liu, Huan; Li, Haimeng; Wang, Hongli; Hao, Ruoshi; Cheng, Xiaofang; Li, Yahong; Yang, Pengcheng; Sahu, Sunil Kumar; Chen, Yaping; Cheng, Le; He, Shuqi; Liu, Ping; Fan, Guangyi; Lu, Haorong; Hu, Guohai; Dong, Wei; Chen, Bin; Jiang, Yuan; Zhang, Yongwei; Xu, Hanhong; Lin, Fei; Slippers, Bernard; Postma, Alisa; Jackson, Matthew; Abate, Birhan Addisie; Tesfaye, Kassahun; Demie, Aschalew Lemma; Bayeleygne, Meseret Destaw; Degefu, Dawit Tesfaye; Chen, Feng; Kuria, Paul K.; Kinyua, Zachary M.; Liu, Tong-Xian; Yang, Huanming; Huang, Fangneng; Liu, Xin; Sheng, Jun; Kang, Le
Date:
2022-07
Abstract:
The fall armyworm (FAW), Spodoptera frugiperda, is a
destructive pest native to America and has recently
become an invasive insect pest in China. Because of its
rapid spread and great risks in China, understanding of
FAW genetic background and pesticide resistance is
urgent and essential to develop effective management
strategies. Here, we assembled a chromosome-level
genome of a male FAW (SFynMstLFR) and compared resequencing
results of the populations from America,
Africa, and China. Strain identification of 163 individuals
collected from America, Africa and China showed that
both C and R strains were found in the American populations,
while only C strain was found in the Chinese
and African populations. Moreover, population genomics
analysis showed that populations from Africa and
China have close relationship with significantly genetic
differentiation from American populations. Taken together,
FAWs invaded into China were most likely originated
from Africa. Comparative genomics analysis
displayed that the cytochrome p450 gene family is
extremely expanded to 425 members in FAW, of which
283 genes are specific to FAW. Treatments of Chinese
populations with twenty-three pesticides showed the variant patterns of transcriptome profiles, and several
detoxification genes such as AOX, UGT and GST specially
responded to the pesticides. These findings will be
useful in developing effective strategies for management
of FAW in China and other invaded areas.
