Abstract:
The effect of neonatal oral supplementation of oleanolic acid (OA) on peroxisome proliferator-activated
receptor gamma
(PPARγ)-1 on glucose homeostasis in high fructose-fed
rats was investigated. Rat pups (7 days old) were randomly assigned to and
randomly administered with control (Distilled water, DW), OA (60 mg/kg), metformin (MET, 500 mg/kg), high fructose solution
(HFS, 20% w/v), OA + HFS, MET + HFS, and treated till postnatal day (PND) 14. The pups were weaned onto a standard diet
on PND 21 up to PND 111 and terminated on PND 112. Glucose derivatives and gene expressions of PPARγ-1 and glucose
transporter type 4 (Glut-4) in the skeletal muscles were determined by using reverse transcription-quantitative
polymerase chain
reaction and gas chromatography-mass
spectrometry, respectively. HFS significantly lowered glucose concentration and showed
the propensity to suppress the expression of PPARγ-1, but not significantly. OA and MET alone significantly increased PPARγ-1
and Glut-4 expressions. There was no significant difference between the OA and OA + HFS for PPARγ-1 and Glut-4 expressions,
although OA expressions were always higher than that of the OA + HFS group. An elevated level of glucose-6-phosphate
was
observed in OA, MET, and OA + HFS groups. Ribose-5-phosphate
was significantly higher in OA and MET groups than the
control. Ribose-5-phosphate
was also significantly high in OA-treated
group compared with OA + HFS. It is concluded that the
neonatal supplementation with OA could help to improve the activity of PPARγ in reducing the burden of metabolic diseases.
