dc.contributor.author |
Kirschel, Alexander N.G.
|
|
dc.contributor.author |
Nwankwo, Emmanuel C.
|
|
dc.contributor.author |
Pierce, Daniel K.
|
|
dc.contributor.author |
Lukhele, Sifiso M.
|
|
dc.contributor.author |
Moysi, Michaella
|
|
dc.contributor.author |
Ogolowa, Bridget O.
|
|
dc.contributor.author |
Hayes, Sophia C.
|
|
dc.contributor.author |
Monadjem, Ara
|
|
dc.contributor.author |
Brelsford, Alan
|
|
dc.date.accessioned |
2021-04-15T06:14:15Z |
|
dc.date.issued |
2020-12 |
|
dc.description |
DATA AVAILABILITY STATEMENT : The Pogoniulus pusillus genome assembly has been deposited at NCBI SRA in BioProject PRJNA630018, with DDRAD sequencing reads under BioProject PRJNA666541. The master VCF file and gemma and R Code have been deposited in the Dryad Digital Repository (https://doi-org.uplib.idm.oclc.org/10.5061/dryad.jm63xsj87). |
en_ZA |
dc.description.abstract |
It has long been of interest to identify the phenotypic traits that mediate reproductive isolation between related species, and more recently, the genes that underpin them. Much work has focused on identifying genes associated with animal colour, with the candidate gene CYP2J19 identified in laboratory studies as the ketolase converting yellow dietary carotenoids to red ketocarotenoids in birds with red pigments. However, evidence that CYP2J19 explains variation between red and yellow feather coloration in wild populations of birds is lacking. Hybrid zones provide the opportunity to identify genes associated with specific traits. Here we investigate genomic regions associated with colour in red‐fronted and yellow‐fronted tinkerbirds across a hybrid zone in southern Africa. We sampled 85 individuals, measuring spectral reflectance of forecrown feathers and scoring colours from photographs, while testing for carotenoid presence with Raman spectroscopy. We performed a genome‐wide association study to identify associations with carotenoid‐based coloration, using double‐digest RAD sequencing aligned to a short‐read whole genome of a Pogoniulus tinkerbird. Admixture mapping using 104,933 single nucleotide polymorphisms (SNPs) identified a region of chromosome 8 that includes CYP2J19 as the only locus with more than two SNPs significantly associated with both crown hue and crown score, while Raman spectra provided evidence of ketocarotenoids in red feathers. Asymmetric backcrossing in the hybrid zone suggests that yellow‐fronted females mate more often with red‐fronted males than vice versa. Female red‐fronted tinkerbirds mating assortatively with red‐crowned males is consistent with the hypothesis that converted carotenoids are an honest signal of quality. |
en_ZA |
dc.description.department |
Mammal Research Institute |
en_ZA |
dc.description.department |
Zoology and Entomology |
en_ZA |
dc.description.embargo |
2021-10-15 |
|
dc.description.librarian |
hj2021 |
en_ZA |
dc.description.sponsorship |
FP7 Marie Curie Reintegration Grant, a University of Cyprus Research Grant, an AG Leventis Foundation grant and by the AP Leventis Ornithological Research Institute, Jos, Nigeria. |
en_ZA |
dc.description.uri |
http://wileyonlinelibrary.com/journal/mec |
en_ZA |
dc.identifier.citation |
Kirschel ANG, Nwankwo EC, Pierce
DK, et al. CYP2J19 mediates carotenoid colour introgression
across a natural avian hybrid zone. Molecular Ecology 2020;29:4970–
4984. https://doi.org/10.1111/mec.15691. |
en_ZA |
dc.identifier.issn |
0962-1083 (print) |
|
dc.identifier.issn |
1365-294X (online) |
|
dc.identifier.other |
10.1111/mec.15691 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/79451 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Wiley |
en_ZA |
dc.rights |
© 2020 John Wiley & Sons Ltd. This is the pre-peer reviewed version of the following article : CYP2J19 mediates carotenoid colour introgression
across a natural avian hybrid zone. Molecular Ecology 2020;29:4970–
4984. https://doi.org/10.1111/mec.15691. The definite version is available at : http://wileyonlinelibrary.com/journal/mec. |
en_ZA |
dc.subject |
Introgressive hybridization |
en_ZA |
dc.subject |
Plumage coloration |
en_ZA |
dc.subject |
Pogoniulus tinkerbirds |
en_ZA |
dc.subject |
Raman spectroscopy |
en_ZA |
dc.subject |
Sexual selection |
en_ZA |
dc.subject |
Speciation |
en_ZA |
dc.subject |
Single nucleotide polymorphism (SNP) |
en_ZA |
dc.title |
CYP2J19 mediates carotenoid colour introgression across a natural avian hybrid zone |
en_ZA |
dc.type |
Postprint Article |
en_ZA |