Genetic diversity of ‘Candidatus Liberibacter africanus’ in South Africa based on microsatellite markers

Show simple item record Roberts, Ronel Lin, Hong Pietersen, Gerhard 2021-04-12T09:57:06Z 2021-04-12T09:57:06Z 2021-02
dc.description.abstract Citrus Greening disease (CG) in South Africa (SA) is associated with the fastidious bacterium ‘Candidatus Liberibacter africanus’ (Laf). It has been observed that Laf isolates obtained from different geographic localities in SA differed in the rate of transmission during grafting experiments leading to the hypothesis that genetic variation of Laf may exist in this country. To determine this, 167 Laf isolates obtained from Limpopo, North West, Mpumalanga and the Western Cape were subjected to microsatellite analyses, using four polymorphic markers. From UPGMA and STRUCTURE analysis, it was shown that most sources belong to one of two major genetic groups of Laf and these comprise 25 distinct haplotypes. Four samples included within this study did not group with these two major groups, suggesting a potential third and fourth genetic group of Laf being present, which can be validated by further sampling. Results further indicate that Laf populations in SA are formed by geographic locality. The high genetic diversity observed for Laf within this study is consistent with the hypothesis that Laf originated on the African continent, warranting further genetic analysis of Laf populations from Africa. This is the first study to unveil the genetic diversity of Laf. en_ZA
dc.description.department Biochemistry en_ZA
dc.description.department Genetics en_ZA
dc.description.department Microbiology and Plant Pathology en_ZA
dc.description.librarian hj2021 en_ZA
dc.description.sponsorship Citrus Research International and the European Union’s Horizon 2020 research and innovation programme. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Roberts, R., Lin, H. & Pietersen, G. Genetic diversity of ‘Candidatus Liberibacter africanus’ in South Africa based on microsatellite markers. European Journal of Plant Pathology 159, 259–268 (2021). en_ZA
dc.identifier.issn 0929-1873 (print)
dc.identifier.issn 1573-8469 (online)
dc.identifier.other 10.1007/s10658-020-02180-w
dc.language.iso en en_ZA
dc.publisher Springer en_ZA
dc.rights © The Author(s) 2021. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License. en_ZA
dc.subject Citrus greening en_ZA
dc.subject Candidatus Liberibacter africanus en_ZA
dc.subject Microsatellite en_ZA
dc.subject Population en_ZA
dc.title Genetic diversity of ‘Candidatus Liberibacter africanus’ in South Africa based on microsatellite markers en_ZA
dc.type Postprint Article en_ZA

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