Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes

Show simple item record Victorica, Matias Romero Soria, Marcelo A. Batista-Garcia, Ramon Alberto Ceja-Navarro, Javier A. Vikram, Surendra Ortiz, Maximiliano Ontanon, Ornella Ghio, Silvina Martínez-Avila, Liliana Quintero García, Omar Jasiel Etcheverry, Clara Campos, Eleonora Cowan, Don A. Arneodo, Joel Talia, Paola M. 2021-04-08T12:28:41Z 2021-04-08T12:28:41Z 2020-03-02
dc.description.abstract In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation. Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances. However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation. The C. fulviceps gut microbiome was enriched specifically in genes coding for debranching- and oligosaccharide-degrading enzymes. These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species. To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4- xylanase, Xyl10E, was cloned and expressed in Escherichia coli. Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10. These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications. en_ZA
dc.description.department Biochemistry en_ZA
dc.description.department Genetics en_ZA
dc.description.department Microbiology and Plant Pathology en_ZA
dc.description.librarian am2021 en_ZA
dc.description.sponsorship CONICET Fellowships, the Instituto Nacional de Tecnología Agropecuaria (INTA), Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) Proyectos de Investigación Científica y Tecnológica (PICT), Conacyt and the Conacyt Fellowships. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Romero Victorica, M., Soria, M.A., Batista-García, R.A. et al. Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes. Scientific Reports 10, 3864 (2020). en_ZA
dc.identifier.issn 2045-2322 (online)
dc.identifier.other 10.1038/s41598-020-60850-5
dc.language.iso en en_ZA
dc.publisher Nature Publishing Group en_ZA
dc.rights © The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License. en_ZA
dc.subject Feeding habits en_ZA
dc.subject Termite en_ZA
dc.subject Cortaritermes fulviceps en_ZA
dc.subject Nasutitermes aquilinus en_ZA
dc.subject Metagenomics en_ZA
dc.subject Next-generation sequencing (NGS) en_ZA
dc.title Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes en_ZA
dc.type Article en_ZA

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