Human immunodeficiency virus still remains the leading cause of death globally
including women of child-bearing age. The rate of AIDS-related death has
significantly declined since the introduction of antiretroviral treatment and other
non-medical interventions such as the distribution and use of condoms. The
introduction of antiretroviral treatment has however led to insulin resistance amongst users. Clustered regularly interspaced short palindromic repeats (CRISPR)
CRISPR-associated nuclease 9 (Cas) has been used to knockout NFκB to
understand the pathway at which antiretroviral treatment causes insulin resistance.
Heteroduplex mobility assay has shown that CRISPR-Cas9 knock out the gene of
interest. These results have played a foundation in understanding how CRISPR-Cas9 can be integrated and utilized in medical research.
Dissertation (MSc (Chemical Pathology))--University of Pretoria, 2020.