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dc.contributor.author | Kolo, Agatha Onyemowo![]() |
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dc.contributor.author | Collins, Nicola E.![]() |
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dc.contributor.author | Brayton, Kelly A.![]() |
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dc.contributor.author | Chaisi, Mamohale E.![]() |
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dc.contributor.author | Blumberg, Lucille Hellen![]() |
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dc.contributor.author | Frean, John![]() |
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dc.contributor.author | Gall, Cory A.![]() |
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dc.contributor.author | Wentzel, Jeanette Maria![]() |
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dc.contributor.author | Wills-Berriman, Samantha![]() |
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dc.contributor.author | De Boni, Liesl![]() |
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dc.contributor.author | Weyer, Jacqueline![]() |
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dc.contributor.author | Rossouw, Jennifer![]() |
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dc.contributor.author | Oosthuizen, Marinda C.![]() |
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dc.date.accessioned | 2021-04-06T10:27:40Z | |
dc.date.available | 2021-04-06T10:27:40Z | |
dc.date.issued | 2020-11 | |
dc.description | Supplementary Materials: Table S1: List of rodent species captured from the three habitat areas, Table S2: Origin and list of samples on which circular consensus sequencing (CCS) and multilocus gene sequencing were performed, Table S3: GenBank accession numbers of sequences used in the phylogenetic analysis of the Anaplasma species, Table S4: Sample information for the 16S rRNA, gltA, msp4 and ankA sequences generated in this study, Figure S1: Alignment of msp2 sequences from A. platys and A. phagocytophilum, Figure S2: Representative rarefaction curves from samples tested, Figure S3: Alignment of msp4 sequences from several species of Anaplasma, Figure S4: Alignment of ankA sequences from a few Anaplasma species showing the region that was amplified in this study. | en_ZA |
dc.description.abstract | DNA samples from 74 patients with non-malarial acute febrile illness (AFI), 282 rodents, 100 cattle, 56 dogs and 160 Rhipicephalus sanguineus ticks were screened for the presence of Anaplasma phagocytophilum DNA using a quantitative PCR (qPCR) assay targeting the msp2 gene. The test detected both A. phagocytophilum and Anaplasma sp. SA/ZAM dog DNA. Microbiome sequencing confirmed the presence of low levels of A. phagocytophilum DNA in the blood of rodents, dogs and cattle, while high levels of A. platys and Anaplasma sp. SA/ZAM dog were detected in dogs. Directed sequencing of the 16S rRNA and gltA genes in selected samples revealed the presence of A. phagocytophilum DNA in humans, dogs and rodents and highlighted its importance as a possible contributing cause of AFI in South Africa. A number of recently described Anaplasma species and A. platys were also detected in the study. Phylogenetic analyses grouped Anaplasma sp. SA/ZAM dog into a distinct clade, with sufficient divergence from other Anaplasma species to warrant classification as a separate species. Until appropriate type-material can be deposited and the species is formally described, we will refer to this novel organism as Anaplasma sp. SA dog. | en_ZA |
dc.description.department | Medical Virology | en_ZA |
dc.description.department | Veterinary Tropical Diseases | en_ZA |
dc.description.librarian | hj2021 | en_ZA |
dc.description.sponsorship | Belgian Directorate General for Development Co-operation Framework (ITM/DGCD), and the South African National Research Foundation. | en_ZA |
dc.description.uri | http://www.mdpi.com/journal/microorganisms | en_ZA |
dc.identifier.citation | Kolo, A.O., Collins, N.E., Brayton, K.A. et al. 2020, 'Anaplasma phagocytophilum and other Anaplasma spp. in various hts in the Mnisi Community, Mpumalanga Province, South Africa', Microorganisms, vol. 8, no. 11, art. 1812, pp. 1-21; https://doi.org/10.3390/microorganisms8111812. | en_ZA |
dc.identifier.issn | 2076-2607 (online) | |
dc.identifier.other | 10.3390/microorganisms8111812 | |
dc.identifier.uri | http://hdl.handle.net/2263/79314 | |
dc.language.iso | en | en_ZA |
dc.publisher | MDPI | en_ZA |
dc.rights | © 2020 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | en_ZA |
dc.subject | Anaplasma phagocytophilum | en_ZA |
dc.subject | Anaplasma spp. | en_ZA |
dc.subject | Rural population | en_ZA |
dc.subject | Various hosts | en_ZA |
dc.subject | Rhipicephalus sanguineus | en_ZA |
dc.subject | Bacterial community | en_ZA |
dc.subject | Genetic variation | en_ZA |
dc.subject.other | Veterinary science articles SDG-03 | en_ZA |
dc.subject.other | SDG-03: Good health and well-being | |
dc.title | Anaplasma phagocytophilum and other Anaplasma spp. in various hosts in the Mnisi Community, Mpumalanga Province, South Africa | en_ZA |
dc.type | Article | en_ZA |