Molecular detection of rabies lyssaviruses from dogs in Southeastern Nigeria : evidence of transboundary transmission of rabies in West Africa

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dc.contributor.author Eze, Ukamaka U.
dc.contributor.author Ngoepe, Ernest C.
dc.contributor.author Anene, Boniface M.
dc.contributor.author Ezeokonkwo, Romanus C.
dc.contributor.author Nwosuh, Chika I.
dc.contributor.author Sabeta, Claude Taurai
dc.date.accessioned 2021-02-15T08:41:38Z
dc.date.available 2021-02-15T08:41:38Z
dc.date.issued 2020-01-23
dc.description.abstract Despite being the first country to register confirmed cases of Mokola and Lagos bat lyssaviruses (two very distant lyssaviruses), knowledge gaps, particularly on the molecular epidemiology of lyssaviruses, still exist in Nigeria. A total of 278 specimens were collected from dogs in southeastern Nigeria between October 2015 and July 2016, and 23 (8.3%) of these tested positive for lyssaviruses with the direct fluorescent antibody test (DFA). The lyssaviruses were genetically characterized by amplifying the highly conserved nucleoprotein (N) gene of the rabies lyssaviruses (RABVs) of the viral genome. Phylogenetic analyses of the nucleotide sequences showed that all the RABV sequences in this study were of the Africa-2 lineage. Our results demonstrated that transboundary transmission of rabies lyssavirus is a key event, given that one of the RABV sequences (MN196576) clustered with rabies variants from neighboring Niger Republic. Furthermore, three RABVs from dogs from Anambra State clustered separately forming a novel and distinct group. Our results demonstrated that transboundary transmission of RABLVs is a key driver in the spread of rabies in West Africa. In order for the successful control of this zoonotic disease, a multinational stepwise surveillance and elimination of rabies in Africa by 2030 is probably the solution for regional elimination. en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.librarian am2021 en_ZA
dc.description.sponsorship The Tertiary Educational Trust Fund (TETFund) of the Nigerian government through University of Nigeria IBR and Bench Space Intervention (TETFUND/DESS/UNI/NSUKKA/RP/VOL.V) and also the ARC-OVI National Assets [P10000029] Onderstepoort Veterinary Institute, South Africa. en_ZA
dc.description.uri http://www.mdpi.com/journal/viruses en_ZA
dc.identifier.citation Eze, U.U., Ngoepe, E.C., Anene, B.M. et al. 2020, 'Molecular detection of rabies lyssaviruses from dogs in Southeastern Nigeria : evidence of transboundary transmission of rabies in West Africa', Viruses, vol. 12, no. 2, art. 134, pp. 1-14. en_ZA
dc.identifier.issn 1999-4915 (online)
dc.identifier.other 10.3390/v12020134
dc.identifier.uri http://hdl.handle.net/2263/78555
dc.language.iso en en_ZA
dc.publisher MDPI Publishing en_ZA
dc.rights © 2020 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. en_ZA
dc.subject Rabies lyssavirus en_ZA
dc.subject Molecular characterization en_ZA
dc.subject Southeastern Nigeria en_ZA
dc.subject Transboundary transmission en_ZA
dc.subject Dogs (Canis familiaris) en_ZA
dc.subject Rabies lyssavirus (RABV) en_ZA
dc.title Molecular detection of rabies lyssaviruses from dogs in Southeastern Nigeria : evidence of transboundary transmission of rabies in West Africa en_ZA
dc.type Article en_ZA


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