dc.contributor.advisor |
Crafford, Jan Ernst |
|
dc.contributor.coadvisor |
Guthrie, Alan John |
|
dc.contributor.postgraduate |
Jacobs, Nicholaas Hendrik |
|
dc.date.accessioned |
2020-11-04T15:10:11Z |
|
dc.date.available |
2020-11-04T15:10:11Z |
|
dc.date.created |
20/04/22 |
|
dc.date.issued |
2019 |
|
dc.description |
Dissertation (MSc)--University of Pretoria, 2019. |
|
dc.description.abstract |
Recent case reports have suggested an increase in cases where dogs have been
affected by African horse sickness virus (AHSV). Contrary to historic findings,
several cases have been reported where dogs became infected without any
evidence of contact with or consumption of infected horses or their products.
This was a prospective study to determine the prevalence of specific antibody and
nucleic acid to AHSV and Equine encephalosis virus (EEV) in a high risk, isolated
dog population during the high vector period in an endemic area. Dogs were kept in
open kennels in close proximity to horses, sheep and cattle. Dogs in this population
have historically been diagnosed with clinical AHS. Blood samples were collected
on a monthly interval from February to June 2019. Antibody to AHSV was detected
using an indirect ELISA while antibody to EEV was detected using a competitive
ELISA. Infection rates were determined by demonstrating viral nucleic acids by RTqPCR.
All of the 37 sampled dogs remained negative for RNA to both AHSV and
EEV for the duration of the study. This indicates that there were no equine orbiviruses circulating in this dog population and is consistent with the absence of
any clinical signs during the study period. This is also in line with the absence of any
new AHS case reports in dogs from the area. Low levels of antibody were detected
at some time points indicating transient exposure without viremia or due to crossreactions
with unknown antigens. |
|
dc.description.availability |
Unrestricted |
|
dc.description.degree |
MSc |
|
dc.description.department |
Veterinary Tropical Diseases |
|
dc.identifier.citation |
Jacobs, NH 2019, Seroprevalence and infection rates for equine orbivirus infections in dogs at Onderstepoort, South Africa, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/76863> |
|
dc.identifier.other |
A2020 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/76863 |
|
dc.language.iso |
en |
|
dc.publisher |
University of Pretoria |
|
dc.rights |
© 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
|
dc.subject |
UCTD |
|
dc.title |
Seroprevalence and infection rates for equine orbivirus infections in dogs at Onderstepoort, South Africa |
|
dc.type |
Dissertation |
|