A method for the in vitro study of drug transfer across ruminal epithelium

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Authors

Jenkins, W.L.
Davis, L.E.

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AOSIS OpenJournals

Abstract

A simple method for the in vitro study of drug diffusion across ruminal epithelium is described. The characteristics of the isolated membrane were defined by studies of ketone production from butyrate, histological studies, phenolsulphonthalein penetration and permeability to pentobarbital, antipyrine and tetraethylammonium. The preparation was found to be suitable for studies of less than 12 hours duration; after that time the integrity of the membrane as a barrier was lost due to degenerative changes. The method had a primary advantage in that experimental variables could be rigorously controlled.

Description

This article is based as part of a dissertation submitted by the senior author' to the graduate Faculty of the University of Missouri in partial fulfillment of the requirements for the degree of Doctor of Philosophy.

Keywords

In vitro, Drug, Ruminal epithelium, Membrane

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Citation

Jenkins, W.L. & Davis, L.E. 1975, 'A method for the in vitro study of drug transfer across ruminal epithelium', Journal of the South African Veterinary Association, vol. 46, no. 2, pp. 153-160.