dc.contributor.author |
Wallace, David Brian
|
|
dc.contributor.author |
Mather, A.
|
|
dc.contributor.author |
Kara, Pravesh Deepak
|
|
dc.contributor.author |
Naicker, Leeann
|
|
dc.contributor.author |
Mokoena, Nobalanda B.
|
|
dc.contributor.author |
Pretorius, A.
|
|
dc.contributor.author |
Nefefe, T.
|
|
dc.contributor.author |
Thema, N.
|
|
dc.contributor.author |
Babiuk, Shawn
|
|
dc.date.accessioned |
2020-10-09T12:05:31Z |
|
dc.date.available |
2020-10-09T12:05:31Z |
|
dc.date.issued |
2020-05-19 |
|
dc.description.abstract |
Lumpy skin disease and Rift Valley fever are two high-priority livestock diseases which
have the potential to spread into previously free regions through animalmovement and/or
vectors, as well as intentional release by bioterrorists. Since the distribution range of
both diseases is similar in Africa, it makes sense to use a bivalent vaccine to control
them. This may lead to the more consistent and sustainable use of vaccination against
Rift Valley fever through a more cost-effective vaccine. In this study, a recombinant
lumpy skin disease virus was constructed in which the thymidine kinase gene was
used as the insertion site for the Gn and Gc protective glycoprotein genes of Rift Valley
fever virus using homologous recombination. Selection markers, the enhanced green
fluorescent protein and Escherichia coli guanidine phosphoribosyl transferase (gpt), were
used for selection of recombinant virus and in amanner enabling a second recombination
event to occur upon removal of the gpt selection-pressure allowing the removal of both
marker genes in the final product. This recombinant virus, LSD-RVF.mf, was selected
to homogeneity, characterized and evaluated in cattle as a vaccine to show protection
against both lumpy skin disease and Rift Valley fever in cattle. The results demonstrate
that the LSD-RVF.mf is safe, immunogenic and can protect cattle against both diseases. |
en_ZA |
dc.description.department |
Veterinary Tropical Diseases |
en_ZA |
dc.description.librarian |
am2020 |
en_ZA |
dc.description.sponsorship |
This work was generously supported by the GALVmed
organization (recombinant virus construction, selection, and
characterization) (under grant no. 710/CAP/07/003) and the
Canadian International Development Research Centre in
collaboration with Global Affairs Canada, within the Canadian
International Food Security Research Fund (CIFSRF) Grant no.
107848-002 (cattle trial). IDRC have also kindly agreed to pay
the APF. |
en_ZA |
dc.description.uri |
https://www.frontiersin.org/journals/veterinary-science# |
en_ZA |
dc.identifier.citation |
Wallace DB, Mather A, Kara PD,
Naicker L, Mokoena NB, Pretorius A,
Nefefe T, Thema N and Babiuk S
(2020) Protection of Cattle Elicited
Using a Bivalent Lumpy Skin Disease
Virus-Vectored Recombinant Rift
Valley Fever Vaccine.
Frontiers in Veterinary Science 7:256.
DOI: 10.3389/fvets.2020.00256. |
en_ZA |
dc.identifier.issn |
2297-1769 (online) |
|
dc.identifier.other |
10.3389/fvets.2020.00256 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/76419 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Frontiers Media |
en_ZA |
dc.rights |
© 2020 Wallace, Mather, Kara, Naicker, Mokoena, Pretorius, Nefefe,
Thema and Babiuk. This is an open-access article distributed under the terms
of the Creative Commons Attribution License (CC BY). |
en_ZA |
dc.subject |
Recombination |
en_ZA |
dc.subject |
Vaccine |
en_ZA |
dc.subject |
Cattle |
en_ZA |
dc.subject |
Rift Valley fever (RVF) |
en_ZA |
dc.subject |
Lumpy skin disease (LSD) |
en_ZA |
dc.title |
Protection of cattle elicited using a bivalent lumpy skin disease virus-vectored recombinant Rift Valley fever vaccine |
en_ZA |
dc.type |
Article |
en_ZA |