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dc.contributor.author | Berbers, Bas![]() |
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dc.contributor.author | Ceyssens, Pieter-Jan![]() |
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dc.contributor.author | Bogaerts, Pierre![]() |
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dc.contributor.author | Vanneste, Kevin![]() |
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dc.contributor.author | Roosens, Nancy H. C.![]() |
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dc.contributor.author | Marchal, Kathleen![]() |
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dc.contributor.author | De Keersmaecker, Sigrid C.J.![]() |
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dc.date.accessioned | 2020-09-29T15:17:57Z | |
dc.date.available | 2020-09-29T15:17:57Z | |
dc.date.issued | 2020-08-11 | |
dc.description | Supplementary material: Figure S1: Mauve alignment between the reconstructed mcr-1 plasmid of assemblies 11–14. Figure S2: Visualization of hybrid assemblies of isolates COL20160015 and S15BD05371 from Flongle runs 1 (a), 2 (b), 3 (c), 4 (d) and 5 (e). Figure S3: Visualization of MinION assemblies of isolates COL20160015 and S15BD05371 from Flongle runs 1 (a), 2 (b), 3 (c), 4 (d) and 5 (e). Figure S4: Visualization of hybrid assemblies of isolates S16BD08730 (a), S18BD03994 (b), S18BD00864 (c) and S18BD05011 (d). Table S1: Antimicrobial resistance genes and plasmid replicons detected with ResFinder and PlasmidFinder in whole-genome MiSeq, MinION and hybrid assembly of isolate COL20160015 extracted with Genomic Tip 100. Table S2: Antimicrobial resistance genes and plasmid replicons detected with ResFinder and PlasmidFinder in whole-genome MiSeq, MinION and hybrid assembly of isolate R274 extracted with Genomic Tip 100. Table S3: Antimicrobial resistance genes and plasmid replicons detected with ResFinder and PlasmidFinder in whole-genome MiSeq, MinION and hybrid assembly of the conjugated isolate R274 extracted with Genomic Tip 100 with all chromosomal reads (mapped to CP000948.1) filtered out prior to assembly. Table S4: Quality parameters of the extracted DNA of isolate S15BD05371 used for sequencing Table S5: The most similar sequences from the NCBI nt database to the incomplete contigs in the hybrid assemblies. Table S6: Antimicrobial resistance genes detected with ResFinder in whole-genome MiSeq, MinION and hybrid assembly of isolate S15BD05371 extracted with MagCore. Table S7: Antimicrobial resistance genes detected with ResFinder in MiSeq, MinION and hybrid assembly of isolate S15BD05371 extracted with Genomic Tip500 with plasmid extraction bu ers. Table S8: Antimicrobial resistance genes detected with ResFinder in MiSeq, MinION and hybrid assembly of isolate S15BD05371 extracted with a phenol chloroform plasmid extraction. Table S9: Antimicrobial resistance genes detected with ResFinder in MiSeq, MinION and hybrid assembly of isolate S15BD05371 extracted with phenol chloroform plasmid extraction followed up by an exonuclease digestion and amplification. Table S10: Antimicrobial resistance genes detected with ResFinder in the hybrid assembly of isolate COL20160015 from Flongle run 1. Table S11: Antimicrobial resistance genes detected with ResFinder in the hybrid assembly of isolate COL20160015 from Flongle run 2. Table S12: Antimicrobial resistance genes detected with ResFinder in the hybrid assembly of isolate COL20160015 from Flongle run 3. Table S13: Antimicrobial resistance genes detected with ResFinder in the hybrid assembly of isolate COL20160015 from Flongle run 4. Table S14: Antimicrobial resistance genes detected with ResFinder in the hybrid assembly of isolate S15BD05371 from Flongle run 5. Table S15: Statistics of all sequencing reads that were used in assemblies of this study. | en_ZA |
dc.description.abstract | Antimicrobial resistance (AMR) is one of the most prominent public health threats. AMR genes localized on plasmids can be easily transferred between bacterial isolates by horizontal gene transfer, thereby contributing to the spread of AMR. Next-generation sequencing (NGS) technologies are ideal for the detection of AMR genes; however, reliable reconstruction of plasmids is still a challenge due to large repetitive regions. This study proposes a workflow to reconstruct plasmids with NGS data in view of AMR gene localization, i.e., chromosomal or on a plasmid. Whole-genome and plasmid DNA extraction methods were compared, as were assemblies consisting of short reads (Illumina MiSeq), long reads (Oxford Nanopore Technologies) and a combination of both (hybrid). Furthermore, the added value of conjugation of a plasmid to a known host was evaluated. As a case study, an isolate harboring a large, low-copy mcr-1-carrying plasmid (>200 kb) was used. Hybrid assemblies of NGS data obtained from whole-genome DNA extractions of the original isolates resulted in the most complete reconstruction of plasmids. The optimal workflow was successfully applied to multidrug-resistant Salmonella Kentucky isolates, where the transfer of an ESBL-gene-containing fragment from a plasmid to the chromosome was detected. This study highlights a strategy including wet and dry lab parameters that allows accurate plasmid reconstruction, which will contribute to an improved monitoring of circulating plasmids and the assessment of their risk of transfer. | en_ZA |
dc.description.department | Genetics | en_ZA |
dc.description.librarian | am2020 | en_ZA |
dc.description.sponsorship | The Ylie project ‘AMRSeq’ (BELSPO) and by the Be READY project (Sciensano RP-PJ). | en_ZA |
dc.description.uri | http://www.mdpi.com/journal/antibiotics | en_ZA |
dc.identifier.citation | Berbers, B., Ceyssens, P.J., Bogaerts, P. et al. 2020, 'Development of an NGS-based workflow for improved monitoring of circulating plasmids in support of risk assessment of antimicrobial resistance gene dissemination', Antibiotics, vol. 9, no. 8, art. 503, pp. 1-29. | en_ZA |
dc.identifier.issn | 2079-6382 (online) | |
dc.identifier.other | 10.3390/antibiotics9080503 | |
dc.identifier.uri | http://hdl.handle.net/2263/76271 | |
dc.language.iso | en | en_ZA |
dc.publisher | MDPI | en_ZA |
dc.rights | © 2020 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | en_ZA |
dc.subject | Plasmids | en_ZA |
dc.subject | Conjugation | en_ZA |
dc.subject | DNA extraction | en_ZA |
dc.subject | MiSeq | en_ZA |
dc.subject | MinION | en_ZA |
dc.subject | Flongle | en_ZA |
dc.subject | Hybrid assembly | en_ZA |
dc.subject | Surveillance | en_ZA |
dc.subject | Mobile elements | en_ZA |
dc.subject | Antimicrobial resistance (AMR) | en_ZA |
dc.subject | Next-generation sequencing (NGS) | en_ZA |
dc.title | Development of an NGS-based workflow for improved monitoring of circulating plasmids in support of risk assessment of antimicrobial resistance gene dissemination | en_ZA |
dc.type | Article | en_ZA |