dc.contributor.author |
Feng, Ye
|
|
dc.contributor.author |
Ramnarine, Varune Rohan
|
|
dc.contributor.author |
Bell, Robert
|
|
dc.contributor.author |
Volik, Stanislav
|
|
dc.contributor.author |
Davicioni, Elai
|
|
dc.contributor.author |
Hayes, Vanessa M.
|
|
dc.contributor.author |
Ren, Shancheng
|
|
dc.contributor.author |
Collins, Colin C.
|
|
dc.date.accessioned |
2020-07-10T15:40:27Z |
|
dc.date.available |
2020-07-10T15:40:27Z |
|
dc.date.issued |
2019-02-18 |
|
dc.description |
Additional file 1: Clinical pathological information of study cohort. |
en_ZA |
dc.description |
Additional file 2: The number of raw and normalized reads for
each taxonomic unit for both metagenome and metatranscriptome. |
en_ZA |
dc.description |
Additional file 3: Comparison of bacterial composition between
metagenome and metatranscriptome. (A) Venn diagram of bacterial
genera identified by metagenome, metatranscriptome and the study by
Yow et al. [13]. (B) The NMDS plot shows that the metagenomic and metatranscriptomic data could be clearly separated in terms of bacterial
composition. Each dot represented a specimen. |
en_ZA |
dc.description |
Additional file 4: Prostatic virome. The heatmap represents the
normalized read counts for the identified viruses. |
en_ZA |
dc.description |
Additional file 5: Detailed annotation and nucleotide sequences of the
10 bacterial genes that have the correlated expression profile with the
eight small RNA genes. |
en_ZA |
dc.description.abstract |
BACKGROUND : Prostate cancer (PCa) is the most common malignant neoplasm among men in many countries.
Since most precancerous and cancerous tissues show signs of inflammation, chronic bacterial prostatitis has been
hypothesized to be a possible etiology. However, establishing a causal relationship between microbial inflammation
and PCa requires a comprehensive analysis of the prostate microbiome. The aim of this study was to characterize
the microbiome in prostate tissue of PCa patients and investigate its association with tumour clinical characteristics
as well as host expression profiles.
RESULTS : The metagenome and metatranscriptome of tumour and the adjacent benign tissues were assessed in 65
Chinese radical prostatectomy specimens. Escherichia, Propionibacterium, Acinetobacter and Pseudomonas were
abundant in both metagenome and metatranscriptome, thus constituting the core of the prostate microbiome. The
biodiversity of the microbiomes could not be differentiated between the matched tumour/benign specimens or
between the tumour specimens of low and high Gleason Scores. The expression profile of ten Pseudomonas genes
was strongly correlated with that of eight host small RNA genes; three of the RNA genes may negatively associate
with metastasis. Few viruses could be identified from the prostate microbiomes.
CONCLUSIONS : This is the first study of the human prostate microbiome employing an integrated metagenomics
and metatranscriptomics approach. In this Chinese cohort, both metagenome and metatranscriptome analyses
showed a non-sterile microenvironment in the prostate of PCa patients, but we did not find links between the
microbiome and local progression of PCa. However, the correlated expression of Pseudomonas genes and human
small RNA genes may provide tantalizing preliminary evidence that Pseudomonas infection may impede metastasis. |
en_ZA |
dc.description.department |
School of Health Systems and Public Health (SHSPH) |
en_ZA |
dc.description.librarian |
am2020 |
en_ZA |
dc.description.sponsorship |
China Scholarship Council, Terry Fox Foundation, Prostate Cancer Canada Team
Grant, National Natural Science Foundation of China. |
en_ZA |
dc.description.uri |
https://bmcgenomics.biomedcentral.com |
en_ZA |
dc.identifier.citation |
Feng, Y., Ramnarine, V.R., Bell, R. et al. 2019, 'Metagenomic and metatranscriptomic
analysis of human prostate microbiota from
patients with prostate cancer', BMC Genomics, vol. 20, art. 146, pp. 1-8. |
en_ZA |
dc.identifier.issn |
1471-2164 (online) |
|
dc.identifier.other |
10.1186/s12864-019-5457-z |
|
dc.identifier.uri |
http://hdl.handle.net/2263/75144 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
BioMed Central |
en_ZA |
dc.rights |
© The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0
International License. |
en_ZA |
dc.subject |
Metagenome |
en_ZA |
dc.subject |
Metatranscriptome |
en_ZA |
dc.subject |
Microbial infection |
en_ZA |
dc.subject |
Prostate cancer |
en_ZA |
dc.subject |
Pseudouridylation |
en_ZA |
dc.title |
Metagenomic and metatranscriptomic analysis of human prostate microbiota from patients with prostate cancer |
en_ZA |
dc.type |
Article |
en_ZA |