Gene expression profiles of the small intestine of village chickens from an Ascaridia galli infested environment

Show simple item record Malatji, Dikeledi Petunia Van Marle-Koster, Este Muchadeyi, F.C. 2020-06-15T09:31:25Z 2020-06-15T09:31:25Z 2019-11
dc.description.abstract Nematodes of the genus Ascaridia are known to infect many species of birds and result in fatal diseases. A. galli damages the intestinal mucosa of chickens leading to blood loss, secondary infection and occasionally the obstruction of small intestines due to high worm burden. This study investigated the gene expression profiles in chickens from two different provinces of South Africa naturally exposed to A. galli infestations and tested either positive or negative for the parasite. The study further investigated gene expression profiles of the A. galli infected duodenum, jejunum and ileum tissues of the small intestines. The A. galli positive intestines displayed hypertrophy of the intestinal villi with accumulation of inflammatory cells and necrosis of the crypts of Lieberkühn glands, lesions that were absent in the uninfected intestines. Total RNA isolated from small intestines of infected and non-infected intestines was sequenced using Illumina HiSeq technology to generate up to 23,856,130 reads. Between any two-way comparisons of the intestines, 277 and 190 transcripts were significantly expressed in Limpopo and KwaZulu-Natal (KZN) chickens, respectively. Gene ontology analysis of the differentially expressed genes (DEGs) revealed an enrichment of genes reported to function in the immune response, defense response, inflammatory response and cell signalling genes. T cell receptor signalling pathways and arachidonic acid metabolism pathways were among the most significantly impacted pathways. Overall, the study provided insights into adaptative mechanisms for chickens extensively raised in parasite infected environments. en_ZA
dc.description.department Animal and Wildlife Sciences en_ZA
dc.description.librarian pm2020 en_ZA
dc.description.sponsorship Tthe Agricultural Research Council-BTP and the NRF under the Zambia/south Africa bilateral Research Program. Dr Malatji received an NRF-DST Professional Development Program research fellowship and University of Pretoria postgraduate support bursary. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Malatji, D.P., Van Marle-Koster, E. & Muchadeyi, F.C. 2019, 'Gene expression profiles of the small intestine of village chickens from an Ascaridia galli infested environment', Veterinary Parasitology: X, vol. 2, art. 100012, pp. 1-12. en_ZA
dc.identifier.issn 2590-1389
dc.identifier.other 10.1016/j.vpoa.2019.100012
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license ( en_ZA
dc.subject Ascaridia galli en_ZA
dc.subject Nematode infections en_ZA
dc.subject Chickens en_ZA
dc.subject Transcriptomes en_ZA
dc.subject Adaptive mechanisms en_ZA
dc.subject South Africa (SA) en_ZA
dc.title Gene expression profiles of the small intestine of village chickens from an Ascaridia galli infested environment en_ZA
dc.type Article en_ZA

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