Activation of a bovine mammary epithelial cell line by ruminant-associated Staphylococcus aureus is lineage dependent

Show simple item record Hoekstra, Jurriaan Rutten, Victor P.M.G. Lam, Theo J.G.M. Van Kessel, Kok P.M. Stegeman, J. Arjan Benedictus, Lindert Koop, Gerrit 2020-05-12T14:03:33Z 2020-05-12T14:03:33Z 2019-12-12
dc.description.abstract Bovine mastitis is a costly disease to the dairy industry and intramammary infections (IMI) with Staphylococcus aureus are a major cause of mastitis. Staphylococcus aureus strains responsible for mastitis in cattle predominantly belong to ruminant-associated clonal complexes (CCs). Recognition of pathogens by bovine mammary epithelial cells (bMEC) plays a key role in activation of immune responsiveness during IMI. However, it is still largely unknown to what extent the bMEC response di ers according to S. aureus CC. The aim of this study was to determine whether ruminant-associated S. aureus CCs di erentially activate bMEC. For this purpose, the immortalized bMEC line PS was stimulated with S. aureus mastitis isolates belonging to four di erent clonal complexes (CCs; CC133, CC479, CC151 and CC425) and interleukin 8 (IL-8) release was measured as indicator of activation. To validate our bMEC model, we first stimulated PS cells with genetically modified S. aureus strains lacking (protein A, wall teichoic acid (WTA) synthesis) or expressing (capsular polysaccharide (CP) type 5 or type 8) factors expected to a ect S. aureus recognition by bMEC. The absence of functional WTA synthesis increased IL-8 release by bMEC in response to bacterial stimulation compared to wildtype. In addition, bMEC released more IL-8 after stimulation with S. aureus expressing CP type 5 compared to CP type 8 or a strain lacking CP expression. Among the S. aureus lineages, isolates belonging to CC133 induced a significantly stronger IL-8 release from bMEC than isolates from the other CCs, and the IL-8 response to CC479 was higher compared to CC151 and CC425. Transcription levels of IL-8, tumor necrosis factor alpha (TNF ), serum amyloid A3 (SAA3), Toll-like receptor (TLR)-2 and nuclear factor B (NF- B) in bMEC after bacterial stimulation tended to follow a similar pattern as IL-8 release, but there were no significant di erences between the CCs. This study demonstrates a di erential activation of bMEC by ruminant-associated CCs of S. aureus, which may have implications for the severity of mastitis during IMI by S. aureus belonging to these lineages. en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.librarian am2020 en_ZA
dc.description.uri en_ZA
dc.identifier.citation Hoekstra, J., Rutten, V.P.M.G., Lam, T.J.G.M. et al. 2019, 'Activation of a bovine mammary epithelial cell line by ruminant-associated Staphylococcus aureus is lineage dependent', Microorganisms, vol. 7, no. 12, art. 688, pp. 1-12. en_ZA
dc.identifier.issn 2076-2607 (online)
dc.identifier.other 10.3390/microorganisms7120688
dc.language.iso en en_ZA
dc.publisher MDPI en_ZA
dc.rights © 2019 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. en_ZA
dc.subject Staphylococcus aureus en_ZA
dc.subject Clonal complexes en_ZA
dc.subject Mastitis en_ZA
dc.subject Immune response en_ZA
dc.subject IL-8 en_ZA
dc.subject Bovine mammary epithelial cells (bMEC) en_ZA
dc.title Activation of a bovine mammary epithelial cell line by ruminant-associated Staphylococcus aureus is lineage dependent en_ZA
dc.type Article en_ZA

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