A clinically important, plasmid-borne antibiotic resistance gene (β-lactamase TEM-116) present in desert soils

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dc.contributor.author Naidoo, Yashini
dc.contributor.author Valverde, Angel
dc.contributor.author Cason, Errol D.
dc.contributor.author Pierneef, Rian
dc.contributor.author Cowan, Don A.
dc.date.accessioned 2020-05-12T11:21:10Z
dc.date.issued 2020-06
dc.description.abstract The exhaustive use of antibiotics in humans, animal farming and other agricultural practices has resulted in the frequent appearance of antibiotic resistant bacteria in human-impacted habitats. However, antibiotic resistance in natural (less-impacted) habitats is less understood. Using shotgun metagenomics we analysed soils from relatively low anthropogenic impact sites across the Namib Desert. We report the presence of a clinically significant extended spectrum β-lactamase (TEM-116), on a ColE1-like plasmid also carrying a metal resistance gene (arsC). The co-occurrence of resistance to antimicrobial drugs and metals encoded on a single mobile genetic element increases the probability of dissemination of these resistance determinants and the potential selection of multiple resistance mechanisms. In addition, the presence of a P7 entero-bacteriophage on the same plasmid, may represent a new vehicle for the propagation of TEM-116 in these soil communities. These findings highlight the role of the environment in the One Health initiative. en_ZA
dc.description.department Biochemistry en_ZA
dc.description.department Genetics en_ZA
dc.description.department Microbiology and Plant Pathology en_ZA
dc.description.embargo 2021-06-01
dc.description.librarian hj2020 en_ZA
dc.description.sponsorship The National Research Foundation (NRF) of South Africa en_ZA
dc.description.uri http://www.elsevier.com/locate/scitotenv en_ZA
dc.identifier.citation Naidoo, Y., Valverde, A., Cason, E.D. et al. 2020, 'A clinically important, plasmid-borne antibiotic resistance gene (β-lactamase TEM-116) present in desert soils', Science of the Total Environment, vol. 719, art. 137497, pp. 1-6. en_ZA
dc.identifier.issn 0048-9697 (print)
dc.identifier.issn 1879-1026 (online)
dc.identifier.other 10.1016/j.scitotenv.2020.137497
dc.identifier.uri http://hdl.handle.net/2263/74543
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2020 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Science of the Total Environment. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Science of the Total Environment, vol. 719, art. 137497, pp. 1-6, 2020. doi : 10.1016/j.scitotenv.2020.137497. en_ZA
dc.subject Antibiotic resistance gene (ARG) en_ZA
dc.subject Desert soils en_ZA
dc.subject Metagenomics en_ZA
dc.subject Low anthropogenic impact en_ZA
dc.subject Namib desert en_ZA
dc.subject Mobile genetic element en_ZA
dc.title A clinically important, plasmid-borne antibiotic resistance gene (β-lactamase TEM-116) present in desert soils en_ZA
dc.type Postprint Article en_ZA


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