Vegetative desiccation tolerance in the resurrection plant Xerophyta humilis has not evolved through reactivation of the seed canonical LAFL regulatory network

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dc.contributor.author Lyall, Rafe
dc.contributor.author Schlebusch, Stephen A.
dc.contributor.author Proctor, Jessica
dc.contributor.author Prag, Mayur
dc.contributor.author Hussey, S.G. (Steven)
dc.contributor.author Ingle, Robert A.
dc.contributor.author Illing, Nicola
dc.date.accessioned 2020-04-29T07:36:14Z
dc.date.available 2020-04-29T07:36:14Z
dc.date.issued 2020-03
dc.description.abstract It has been hypothesised that vegetative desiccation tolerance in resurrection plants evolved via reactivation of the canonical LAFL (i.e. LEC1, ABI3, FUS3 and LEC2) transcription factor (TF) network that activates the expression of genes during the maturation of orthodox seeds leading to desiccation tolerance of the plant embryo in most angiosperms. There is little direct evidence to support this, however, and the transcriptional changes that occur during seed maturation in resurrection plants have not previously been studied. Here we performed de novo transcriptome assembly for Xerophyta humilis, and analysed gene expression during seed maturation and vegetative desiccation. Our results indicate that differential expression of a set of 4205 genes is common to maturing seeds and desiccating leaves. This shared set of genes is enriched for gene ontology terms related to abiotic stress, including water stress and abscisic acid signalling, and includes many genes that are seed-specific in Arabidopsis thaliana and targets of ABI3. However, while we observed upregulation of orthologues of the canonical LAFL TFs and ABI5 during seed maturation, similar to what is seen in A. thaliana, this did not occur during desiccation of leaf tissue. Thus, reactivation of components of the seed desiccation program in X. humilis vegetative tissues likely involves alternative transcriptional regulators. en_ZA
dc.description.department Biochemistry en_ZA
dc.description.department Genetics en_ZA
dc.description.department Microbiology and Plant Pathology en_ZA
dc.description.librarian am2020 en_ZA
dc.description.sponsorship The University of Cape Town, the National Research Foundation via Incentive Funding (UID 103423) and a National Bioinformatics Functional Genomics grant (UID 93697). en_ZA
dc.description.uri https://onlinelibrary.wiley.com/journal/1365313x en_ZA
dc.identifier.citation Lyall, R., Schlebusch, S.A., Proctor, J. et al. 2020, 'Vegetative desiccation tolerance in the resurrection plantXerophyta humilis has not evolved through reactivation ofthe seed canonical LAFL regulatory network', Plant Journal, vol. 101, no. 6, pp. 1349–1367. en_ZA
dc.identifier.issn 0960-7412 (print)
dc.identifier.issn 1365-313X (online)
dc.identifier.other 10.1111/tpj.14596
dc.identifier.uri http://hdl.handle.net/2263/74419
dc.language.iso en en_ZA
dc.publisher Wiley en_ZA
dc.rights © 2019 The Authors. This is an open access article under the terms of the Creative Commons Attribution License. en_ZA
dc.subject Desiccation tolerance en_ZA
dc.subject Resurrection plant en_ZA
dc.subject Seed maturation en_ZA
dc.subject LAFL en_ZA
dc.subject ABI3 en_ZA
dc.subject Xerophyta humilis en_ZA
dc.subject Xerophyta viscosa en_ZA
dc.title Vegetative desiccation tolerance in the resurrection plant Xerophyta humilis has not evolved through reactivation of the seed canonical LAFL regulatory network en_ZA
dc.type Article en_ZA


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