Global DNA methylation profiling in peripheral blood cells of South African women with gestational diabetes mellitus

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dc.contributor.author Dias, Stephanie
dc.contributor.author Adam, Sumaiya
dc.contributor.author Van Wyk, Nastasja
dc.contributor.author Rheeder, Paul
dc.contributor.author Louw, Johan
dc.contributor.author Pheiffer, Carmen
dc.date.accessioned 2020-04-15T08:35:39Z
dc.date.available 2020-04-15T08:35:39Z
dc.date.issued 2019
dc.description.abstract BACKGROUND/OBJECTIVE : Recently, several studies have reported that DNA methylation changes in tissue are reflected in blood, sparking interest in the potential use of global DNA methylation as a biomarker for gestational diabetes mellitus (GDM). This study investigated whether global DNA methylation is associated with GDM in South African women. METHODS : Global DNA methylation was quantified in peripheral blood cells of women with (n = 63) or without (n = 138) GDM using the MDQ1 Imprint® DNA Quantification Kit. RESULTS : Global DNA methylation levels were not different between women with or without GDM and were not associated with fasting glucose or insulin concentrations. However, levels were 18% (p = 0.012) higher in obese compared to non-obese pregnant women and inversely correlated with serum adiponectin concentrations (p = 0.005). DISCUSSION : Contrary to our hypothesis, global DNA methylation was not associated with GDM in our population. These preliminary findings suggest that despite being a robust marker of overall genomic methylation that offers opportunities as a biomarker, global DNA methylation profiling may not offer the resolution required to detect methylation differences in the peripheral blood cells of women with GDM. Moreover, global DNA methylation in peripheral blood cells may not reflect changes in placental tissue. Further studies in a larger sample are required to explore the candidacy of a more targeted approach using gene-specific methylation as a biomarker for GDM in our population. en_ZA
dc.description.department Internal Medicine en_ZA
dc.description.department Obstetrics and Gynaecology en_ZA
dc.description.librarian hj2020 en_ZA
dc.description.sponsorship The National Research Foundation, South Africa and the South African Medical Research Council (SAMRC). en_ZA
dc.description.uri http://www.tandfonline.com/loi/ibmk20 en_ZA
dc.identifier.citation Dias, S., Adam, S., Van Wyk, N. et al. Global DNA methylation profiling in peripheral blood cells of South African women with gestational diabetes mellitus. Biomarkers, vol. 24, no. 3, pp. 225-231, 2019. doi : 10.1080/1354750X.2018.1539770. en_ZA
dc.identifier.issn 1354-750X (print)
dc.identifier.issn 1366-5804 (online)
dc.identifier.other 10.1080/1354750X.2018.1539770
dc.identifier.uri http://hdl.handle.net/2263/74154
dc.language.iso en en_ZA
dc.publisher Taylor and Francis en_ZA
dc.rights © 2019 Informa UK Limited, trading as Taylor & Francis Group. This is an electronic version of an article published in Biomarkers, vol. 24, no. 3, pp. 225-231, 2019. doi : 10.1080/1354750X.2018.1539770. Biomarkers is available online at : http://www.tandfonline.com/loi/ibmk20. en_ZA
dc.subject Gestational diabetes mellitus (GDM) en_ZA
dc.subject Obesity en_ZA
dc.subject South Africa (SA) en_ZA
dc.subject Peripheral blood cells en_ZA
dc.subject Biomarker en_ZA
dc.subject Global DNA methylation en_ZA
dc.title Global DNA methylation profiling in peripheral blood cells of South African women with gestational diabetes mellitus en_ZA
dc.type Postprint Article en_ZA


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