Paramyxo- and coronaviruses in Rwandan bats

Loading...
Thumbnail Image

Authors

Markotter, Wanda
Geldenhuys, Marike
Jansen van Vuren, Petrus
Kemp, Alan
Mortlock, Marinda
Mudakikwa, Antoine
Nel, Louis Hendrik
Nziza, Julius
Paweska, Janusz Tadeusz
Weyer, Jacqueline

Journal Title

Journal ISSN

Volume Title

Publisher

MDPI Publishing

Abstract

A high diversity of corona- and paramyxoviruses have been detected in different bat species at study sites worldwide, including Africa, however no biosurveillance studies from Rwanda have been reported. In this study, samples from bats collected from caves in Ruhengeri, Rwanda, were tested for the presence of corona- and paramyxoviral RNA using reverse transcription PCR assays. Positive results were further characterized by DNA sequencing and phylogenetic analysis. In addition to morphological identification of bat species, we also did molecular confirmation of species identities, contributing to the known genetic database available for African bat species. We detected a novel Betacoronavirus in two Geo roy’s horseshoe bats (Rhinolophus clivosus) bats. We also detected several different paramyxoviral species from various insectivorous bats. One of these viral species was found to be homologous to the genomes of viruses belonging to the Jeilongvirus genus. Additionally, a Henipavirus-related sequence was detected in an Egyptian rousette fruit bat (Rousettus aegyptiacus). These results expand on the known diversity of corona- and paramyxoviruses and their geographical distribution in Africa.

Description

Table S1: Bats collected and tested in this study.

Keywords

Paramyxovirus, Coronavirus, Rwanda, Bat, Surveillance, Caves, Barcoding, Egyptian rousette fruit bat (Rousettus aegyptiacus), Geo roy’s horseshoe bats (Rhinolophus clivosus)

Sustainable Development Goals

Citation

Markotter, W., Geldenhuys, M., Jansen van Vuren, P. et al. 2019, 'Paramyxo- and coronaviruses in Rwandan bats', Tropical Medicine and Infectious Disease', vol. 4, art. 99, pp. 1-11.