A highly specific tool for identification of Xanthomonas vasicola pv. musacearum based on five Xvm-specific coding sequences
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Date
Authors
Nakato, Gloria Valentine
Wicker, Emmanuel
Coutinho, Teresa A.
Mahuku, George
Studholme, David J.
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Xanthomonas vasicola pv. musacearum (Xvm) is a bacterial pathogen responsible
for the economically important Xanthomonas wilt disease on banana and enset
crops in Sub-Saharan Africa. Given that the symptoms are similar to those of
other diseases, molecular diagnosis is essential to unambiguously identify this
pathogen and distinguish it from closely related strains not pathogenic on these
hosts. Currently, Xvm identification is based on polymerase chain reaction (PCR)
with GspDm primers, targeting the gene encoding general secretory protein D.
Experimental results and examination of genomic sequences revealed poor specificity of the GspDm PCR. Here, we present and validate five new Xvmspecific
primers amplifying only Xvm strains.
Description
Keywords
Bioinformatics, Microbiology, Molecular biology, Plant biology, Xanthomonas wilt disease, Banana crops, Enset crops, Bacterial pathogen, Polymerase chain reaction (PCR), Xanthomonas vasicola pv. musacearum (Xvm)
Sustainable Development Goals
Citation
Gloria Valentine Nakato,
Emmanuel Wicker,
Teresa A. Coutinho,
George Mahuku,
David J. Studholme. A highly
specific tool for identification
of Xanthomonas vasicola pv.
musacearum based on five
Xvm-specific coding
sequences.
Heliyon 4 (2018) e01080.
DOI: 10.1016/j.heliyon.2018.e01080.