Genome sequence of Malania oleifera, a tree with great value for nervonic acid production

Show simple item record Xu, Chao-Qun Liu, Hui Zhou, Shan-Shan Zhang, Dong-Xu Zhao, Wei Wang, Sihai Chen, Fu Sun, Yan-Qiang Nie, Shuai Jia, Kai-Hua Jiao, Si-Qian Zhang, Ren-Gang Yun, Quan-Zheng Guan, Wenbin Wang, Xuewen Gao, Qiong Bennetzen, Jeffrey L. Maghuly, Fatemeh Porth, Ilga Van de Peer, Yves Wang, Xiao-Ru Ma, Yongpeng Mao, Jian-Feng 2019-09-18T08:49:50Z 2019-09-18T08:49:50Z 2019-01-24
dc.description.abstract BACKGROUND : Malania oleifera, a member of the Olacaceae family, is an IUCN red listed tree, endemic and restricted to the Karst region of southwest China. This tree’s seed is valued for its high content of precious fatty acids (especially nervonic acid). However, studies on its genetic makeup and fatty acid biogenesis are severely hampered by a lack of molecular and genetic tools. FINDINGS : We generated 51 Gb and 135 Gb of raw DNA sequences, using Pacific Biosciences (PacBio) single-molecule real-time and 10× Genomics sequencing, respectively. A final genome assembly, with a scaffold N50 size of 4.65 Mb and a total length of 1.51 Gb, was obtained by primary assembly based on PacBio long reads plus scaffolding with 10× Genomics reads. Identified repeats constituted ∼82% of the genome, and 24,064 protein-coding genes were predicted with high support. The genome has low heterozygosity and shows no evidence for recent whole genome duplication. Metabolic pathway genes relating to the accumulation of long-chain fatty acid were identified and studied in detail. CONCLUSIONS : Here, we provide the first genome assembly and gene annotation for M. oleifera. The availability of these resources will be of great importance for conservation biology and for the functional genomics of nervonic acid biosynthesis. en_ZA
dc.description.department Biochemistry en_ZA
dc.description.department Genetics en_ZA
dc.description.department Microbiology and Plant Pathology en_ZA
dc.description.librarian am2019 en_ZA
dc.description.sponsorship This study was funded by Fundamental Research Funds for the Central Universities (2018BLCB08), by the construction of the workstation for Academician Bennetzen (2015IC056) from Yunnan Provinical Science and Technology Department, by the Breeding of new variety for Malania oleifera and product development of nervonic acid (2015BB018) from the Science Fund of China’s Yunnan government, and by the Natural hybridization and reproductive isolation among plants with high horticulture values (2018428) from Chinese Academy of Sciences. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Xu et al. 2019, 'Genome sequence of Malania oleifera, a tree with great value for nervonic acid production', GigaScience, vol. 8, no. 2, art. giy164, pp. 1-14. en_ZA
dc.identifier.issn 2047-217X (online)
dc.identifier.other 10.1093/gigascience/giy164
dc.language.iso en en_ZA
dc.publisher Oxford University Press en_ZA
dc.rights © The Author(s) 2019. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. en_ZA
dc.subject De novo genome assembly en_ZA
dc.subject Vulnerable plant en_ZA
dc.subject Malania en_ZA
dc.subject Nervonic acid en_ZA
dc.subject Transcriptomes en_ZA
dc.subject Evolution en_ZA
dc.subject Prediction en_ZA
dc.subject Metabolism en_ZA
dc.subject Transcriptome en_ZA
dc.subject Classification en_ZA
dc.subject Identification en_ZA
dc.subject Annotation en_ZA
dc.subject Gypsy database (GyDB) en_ZA
dc.title Genome sequence of Malania oleifera, a tree with great value for nervonic acid production en_ZA
dc.type Article en_ZA

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