Clinical findings, synovial fluid cytology and growth factor concentrations after intra-articular use of a platelet-rich product in horses with osteoarthritis

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dc.contributor.author Smit, Yolandi
dc.contributor.author Marais, Hendrik J.
dc.contributor.author Thompson, P.N. (Peter N.)
dc.contributor.author Mahne, Arnold Theofilus
dc.contributor.author Goddard, Amelia
dc.date.accessioned 2019-09-18T06:55:20Z
dc.date.available 2019-09-18T06:55:20Z
dc.date.issued 2019-05-23
dc.description The article is based on the thesis of author Yolandi Smit, ‘Clinical signs synovial fluid cytology and growth factor concentrations after intra-articular use of a platelet-rich product in horses with osteoarthritis’ available at https://repository.up.ac.za/handle/2263/67909 (URI: http://hdl.handle.net/2263/67909). en_ZA
dc.description.abstract Osteoarthritis is a common cause of lameness in horses, resulting in poor performance. Intraarticular platelet-rich plasma can deliver a collection of bioactive molecules, such as autologous growth factors and proteins involved in the quality of tissue repair. Horses (n=5) with osteoarthritis affecting antebrachiocarpal, middle carpal or metacarpophalangeal joints, and horses (n=5) without osteoarthritis of the corresponding joints (radiographically free of osteoarthritis), were used for the production of platelet-rich plasma which was subsequently injected into selected joints. Clinical and synovial fluid changes after intra-articular injection of platelet-rich plasma as well as synovial platelet-derived growth factor-BB and transforming growth factor-beta 1 concentration changes were evaluated in these joints and compared between normal joints and joints with osteoarthritis. A gravity filtration system produced a moderately concentrated platelet-rich plasma, representing a 4.7-fold increase in baseline platelet concentration. The synovial effusion score was significantly different between the control joints and joints with osteoarthritis on Day 0 with a higher score in the group with osteoarthritis. Within the control group, the synovial effusion score was significantly higher on Days 1 and 2 compared to Day 0. For both groups, the synovial fluid nucleated cell count, predominantly intact neutrophils, was significantly increased on Days 1 and 2, with no significant difference between groups. The mean synovial platelet-derived growth factor-BB and transforming growth factor-beta 1 concentrations were increased for both groups but significantly lowered in the group with osteoarthritis on Day 1 compared to normal joints. Concentrations for platelet-derived growth factor-BB remained unchanged on Day 5, compared to Day 1, with no significant difference between groups. In conclusion, intra-articular treatment with platelet-rich plasma resulted in increased synovial growth factor concentrations in joints but with lower concentrations in joints with osteoarthritis. A transient inflammatory reaction was seen both clinically as an increase in synovial effusion and cytologically in both normal joints and joints with osteoarthritis. en_ZA
dc.description.department Companion Animal Clinical Studies en_ZA
dc.description.department Production Animal Studies en_ZA
dc.description.librarian am2019 en_ZA
dc.description.sponsorship The article processing fee has been partially funded by the South African Veterinary Association. en_ZA
dc.description.sponsorship The V-PET™ systems were sponsored by the manufacturer Pall Corporation, United States. en_ZA
dc.description.uri http://www.jsava.co.za en_ZA
dc.identifier.citation Smit, Y., Marais, H.J., Thompson, P.T., Mahne, A.T. & Goddard, A., 2019, ‘Clinical findings, synovial fluid cytology and growth factor concentrations after intra-articular use of a platelet-rich product in horses with osteoarthritis’, Journal of the South African Veterinary Association 90(0), a1721. https://DOI.org/10.4102/jsava.v90i0.1721. en_ZA
dc.identifier.issn 1019-9128 (print)
dc.identifier.issn 2224-9435 (online)
dc.identifier.other 10.4102/jsava.v90i0.1721
dc.identifier.uri http://hdl.handle.net/2263/71392
dc.language.iso en en_ZA
dc.publisher AOSIS OpenJournals en_ZA
dc.rights © 2019. The Authors. Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License. en_ZA
dc.subject Osteoarthritis en_ZA
dc.subject Platelet-rich plasma en_ZA
dc.subject Intra-articular en_ZA
dc.subject Joint en_ZA
dc.subject Healthy en_ZA
dc.subject Cytokine en_ZA
dc.subject Injection en_ZA
dc.subject Horse (Equus caballus) en_ZA
dc.subject Mesenchymal stem cells en_ZA
dc.subject Serum amyloid A en_ZA
dc.subject Transforming growth factor beta 1 (TGF-β1) en_ZA
dc.subject Platelet-rich plasma gel (PRP-Gel) en_ZA
dc.title Clinical findings, synovial fluid cytology and growth factor concentrations after intra-articular use of a platelet-rich product in horses with osteoarthritis en_ZA
dc.type Article en_ZA


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