Molecular characteristics and genotypic diversity of enterohaemorrhagic Escherichia coli O157:H7 isolates in Gauteng region, South Africa

Show simple item record Bolukaoto, John Y. Kock, Marleen M. Strydom, Kathy-Anne Mbelle, Nontombi Marylucy Ehlers, M.M. (Marthie Magdaleen) 2019-09-11T12:24:47Z 2019-11
dc.description.abstract Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is one of the major foodborne and waterborne pathogens causing severe diseases and outbreaks worldwide. There is scarcity of EHEC O157:H7 data in South Africa. This study was carried out to determine the molecular characteristics and genotypic diversity of EHEC O157:H7 isolates in the Gauteng region, South Africa. Samples were cultured on selective chromogenic media. Antibiotic susceptibility profile of isolates was determined using the VITEK®-2 automated system. Isolates were characterised using multiplex PCR assays and the genetic diversity was determined using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). A total of 520 samples of which 270 environmental water samples and 250 stool specimens were collected and analysed. Overall, EHEC O157:H7 was recovered from 2.3% (12/520) of samples collected. Environmental water samples and clinical stool specimens showed a prevalence of 4.07% (11/270) and 0.4% (1/250) respectively. Antibiotic susceptibility profile varied from isolates with full susceptibility to isolates with resistance to multiple antibiotics. Most resistance was detected to the penicillins, specifically ampicillin (7/12), amoxicillin (3/12) and piperacillin/Tazobactam (3/12) followed by one of the folate inhibitors, trimethoprim (3/12) and the carbapenems, imipenem and meropenem (2/12) each. Three isolates harboured a combination of Shiga-toxins (Stx)-2, intimin (eae) and enterohaemolysin (hlyA) genes, while two isolates harboured the Stx-1, Stx-2 and hlyA genes. The PFGE performed showed that EHEC O157:H7 isolates were genetically diverse, with two minor pulsotypes and eight singletons. The MLST analysis identified three sequence types (STs) (ST10, ST11 and ST1204) that have been previously reported associated with outbreaks. The STs identified in this study pose a potential public health risk to consumers of untreated environmental water and closed human contacts. There is necessity to enhance surveillance in reducing the propagation of this bacterium which is a public health problem. en_ZA
dc.description.department Medical Microbiology en_ZA
dc.description.embargo 2020-11-20
dc.description.librarian hj2019 en_ZA
dc.description.sponsorship The National Health Laboratory Service (NHLS), National Research Foundation (NRF), Rand Water and University of Pretoria, South Africa. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Bolukaoto, J.Y., Kock, M.M., Strydom, K.-A. et al. 2019, 'Molecular characteristics and genotypic diversity of enterohaemorrhagic Escherichia coli O157:H7 isolates in Gauteng region, South Africa', Science of The Total Environment, vol. 692, pp. 297-304. en_ZA
dc.identifier.issn 0048-9697 (print)
dc.identifier.issn 1879-1026 (online)
dc.identifier.other 10.1016/j.scitotenv.2019.07.119
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2019 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Science of the Total Environment. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Science of the Total Environment, vol. 692, pp. 297-304, 2019. doi : 10.1016/j.scitotenv.2019.07.119. en_ZA
dc.subject Enterohaemorrhagic Escherichia coli (EHEC) en_ZA
dc.subject Environmental water sample en_ZA
dc.subject Genetic diversity en_ZA
dc.subject Virulence gene en_ZA
dc.subject Antimicrobial resistance en_ZA
dc.subject South Africa (SA) en_ZA
dc.subject Antibiotics en_ZA
dc.subject Automation en_ZA
dc.subject Biodiversity en_ZA
dc.subject Electrophoresis en_ZA
dc.subject Genes en_ZA
dc.subject Health risks en_ZA
dc.subject Polymerase chain reaction (PCR) en_ZA
dc.subject Public health en_ZA
dc.subject Escherichia coli O157:H7 en_ZA
dc.subject Bacteria (microorganisms) en_ZA
dc.title Molecular characteristics and genotypic diversity of enterohaemorrhagic Escherichia coli O157:H7 isolates in Gauteng region, South Africa en_ZA
dc.type Postprint Article en_ZA

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