Inherent biophysical stability of foot-and-mouth disease SAT1, SAT2 and SAT3 viruses

Show simple item record Scott, Katherine Anne Maake, Lorens Botha, Elizabeth Theron, Jacques Maree, Francois Frederick 2019-08-13T09:34:25Z 2019-04
dc.description.abstract Foot-and-mouth disease (FMD) virus (FMDV) isolates show variation in their ability to withstand an increase in temperature. The FMDV is surprisingly thermolabile, even though this virus is probably subjected to a strong extracellular selective pressure by heat in hot climate regions where FMD is prevalent. The three SAT serotypes, with their particularly low biophysical stability also only yield vaccines of low protective capacity, even with multiple booster vaccinations. The aim of the study was to determine the inherent biophysical stability of field SAT isolates. To characterise the biophysical stability of 20 SAT viruses from Southern Africa, the thermofluor assay was used to monitor capsid dissociation by the release of the RNA genome under a range of temperature, pH and ionic conditions. The SAT2 and SAT3 viruses had a similar range of thermostability of 48–54 °C. However, the SAT1 viruses had a wider range of thermostability with an 8 °C difference but with many viruses being unstable at 43–46 °C. The thermostable A-serotype A24 control virus had the highest thermostability of 55 °C with some SAT2 and SAT3 viruses of similar thermostability. There was a 10 °C difference between the most unstable SAT virus (SAT1/TAN/2/99) and the highly stable A24 control virus. SAT1 viruses were generally more stable compared to SAT2 and SAT3 viruses at the pH range of 6.7–9.1. The effect of ionic buffers on capsid stability showed that SAT1 and SAT2 viruses had an increased stability of 2–9 °C and 2–6 °C, respectively, with the addition of 1 M NaCl. This is in contrast to the SAT3 viruses, which did not show improved stabilisation after addition of 1 M or 0.5 M NaCl buffers. Some buffers showed differing results dependent on the virus tested, highlighting the need to test SAT viruses with different solutions to establish the most stabilising option for storage of each virus. This study confirms for the first time that more stable SAT field viruses are present in the southern Africa region. This could facilitate the selection of the most stable circulating field strains, for adaptation to cultured BHK-21 cells or manipulation by reverse genetics and targeted mutation to produce improved vaccine master seed viruses. en_ZA
dc.description.department Biochemistry en_ZA
dc.description.department Genetics en_ZA
dc.description.department Microbiology and Plant Pathology en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.embargo 2020-04-15
dc.description.librarian hj2019 en_ZA
dc.description.sponsorship The Vaccine Initiative (ESCP) in South Africa. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Scott, K.A., Maake, L., Botha, E. et al. 2019, 'Inherent biophysical stability of foot-and-mouth disease SAT1, SAT2 and SAT3 viruses', Virus Research, vol. 264, pp. 45-55. en_ZA
dc.identifier.issn 0168-1702 (print)
dc.identifier.issn 1872-7492 (online)
dc.identifier.other 10.1016/j.virusres.2019.02.012
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2019 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Virus Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Virus Research, vol. 264, pp. 45-55, 2019. doi : 10.1016/j.virusres.2019.02.012. en_ZA
dc.subject Stability en_ZA
dc.subject Biophysical en_ZA
dc.subject Foot-and-mouth disease (FMD) en_ZA
dc.subject Foot-and-mouth disease virus (FMDV) en_ZA
dc.subject Southern African Territories (SAT) en_ZA
dc.title Inherent biophysical stability of foot-and-mouth disease SAT1, SAT2 and SAT3 viruses en_ZA
dc.type Postprint Article en_ZA

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