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dc.contributor.author | Wolmarans, Elize | |
dc.contributor.author | Nel, Sulette | |
dc.contributor.author | Durandt, Chrisna | |
dc.contributor.author | Mellet, Juanita | |
dc.contributor.author | Pepper, Michael Sean | |
dc.date.accessioned | 2019-08-08T06:59:15Z | |
dc.date.available | 2019-08-08T06:59:15Z | |
dc.date.issued | 2018-12-06 | |
dc.description.abstract | There is still much to learn about the cells used for cell- and gene-based therapies in the clinical setting. Stem cells are found in virtually all tissues in the human body. As a result, cells isolated from these tissues are a heterogeneous population consisting of various subpopulations including stem cells. Several strategies have been used to isolate and define the subpopulations that constitute these heterogeneous populations, one of which is the side population (SP) assay. SP cells are identified by their ability to efflux a fluorescent dye at a rate that is greater than the main cell population. This elevated rate of dye efflux has been attributed to the expression of members of the ATP-binding cassette (ABC) transporter protein family. SP cells have been identified in various tissues. In this review, we discuss the research to date on SP cells, focussing on SP cells identified in haematopoietic stem cells, adipose-derived stromal cells, and dental pulp. | en_ZA |
dc.description.department | Immunology | en_ZA |
dc.description.department | Oral Pathology and Oral Biology | en_ZA |
dc.description.librarian | am2019 | en_ZA |
dc.description.sponsorship | The South African Medical Research Council in terms of the SAMRC’s Flagship Award Project [SAMRC-RFA-UFSP-01-2013/STEM CELLS], the SAMRC Extramural Unit for Stem Cell Research and Therapy, and the Institute for Cellular and Molecular Medicine of the University of Pretoria. | en_ZA |
dc.description.uri | https://www.hindawi.com/journals/sci | en_ZA |
dc.identifier.citation | Wolmarans, E., Nel, S., Durandt, C., et al. 2018, 'Side population : its use in the study of cellular heterogeneity and as a potential enrichment tool for rare cell populations', Stem Cells International, vol. 2018, art. ID 2472137, pp. 1-7. | en_ZA |
dc.identifier.issn | 1687-966X (print) | |
dc.identifier.issn | 1687-9678 (online) | |
dc.identifier.other | 10.1155/2018/2472137 | |
dc.identifier.uri | http://hdl.handle.net/2263/70911 | |
dc.language.iso | en | en_ZA |
dc.publisher | Hindawi Publishing Corporation | en_ZA |
dc.rights | © 2018 Elize Wolmarans et al. This is an open access article distributed under the Creative Commons Attribution License. | en_ZA |
dc.subject | Human body | en_ZA |
dc.subject | Tissues | en_ZA |
dc.subject | Expression | en_ZA |
dc.subject | Efflux pump | en_ZA |
dc.subject | Stromal cells | en_ZA |
dc.subject | Mesenchymal stem | en_ZA |
dc.subject | In vitro | en_ZA |
dc.subject | Bone marrow | en_ZA |
dc.subject | Stem cells | en_ZA |
dc.subject | Side population (SP) | en_ZA |
dc.subject | ATP-binding cassette (ABC) | en_ZA |
dc.title | Side population : its use in the study of cellular heterogeneity and as a potential enrichment tool for rare cell populations | en_ZA |
dc.type | Article | en_ZA |