Side population : its use in the study of cellular heterogeneity and as a potential enrichment tool for rare cell populations
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Date
Authors
Wolmarans, Elize
Nel, Sulette
Durandt, Chrisna
Mellet, Juanita
Pepper, Michael Sean
Journal Title
Journal ISSN
Volume Title
Publisher
Hindawi Publishing Corporation
Abstract
There is still much to learn about the cells used for cell- and gene-based therapies in the clinical setting. Stem cells are found in
virtually all tissues in the human body. As a result, cells isolated from these tissues are a heterogeneous population consisting of
various subpopulations including stem cells. Several strategies have been used to isolate and define the subpopulations that
constitute these heterogeneous populations, one of which is the side population (SP) assay. SP cells are identified by their ability
to efflux a fluorescent dye at a rate that is greater than the main cell population. This elevated rate of dye efflux has been
attributed to the expression of members of the ATP-binding cassette (ABC) transporter protein family. SP cells have been
identified in various tissues. In this review, we discuss the research to date on SP cells, focussing on SP cells identified in
haematopoietic stem cells, adipose-derived stromal cells, and dental pulp.
Description
Keywords
Human body, Tissues, Expression, Efflux pump, Stromal cells, Mesenchymal stem, In vitro, Bone marrow, Stem cells, Side population (SP), ATP-binding cassette (ABC)
Sustainable Development Goals
Citation
Wolmarans, E., Nel, S., Durandt, C., et al. 2018, 'Side population : its use in the study of cellular heterogeneity and as a potential enrichment tool for rare cell populations', Stem Cells International, vol. 2018, art. ID 2472137, pp. 1-7.