Targeted mutational analysis to unravel the complexity of African horse sickness virus NS3 function in mammalian cells

Show simple item record Ferreira-Venter, Linda Venter, Eudri Theron, Jacques Van Staden, Vida 2019-07-24T09:01:56Z 2019-05
dc.description.abstract The African horse sickness virus non-structural protein 3 (NS3) is involved in the final stages of infection. To gain insight into the function of different NS3 domains, we generated reverse genetics-derived mutants, each expressing a modified version of the protein. A functional comparison of these mutants to the wild-type virus in mammalian cells indicated the variable contribution of the different domains to the cytopathic effect and in ensuring effective virus trafficking and release. The transmembrane domains were determined as essential mediators of NS3 localisation, as the abnormal processing of these mutant proteins resulted in their nuclear localisation and interaction with NS1. NS3 cytoplasmic domain disruptions resulted in increased cytosolic virus particle accumulation and abnormal virion tethering to plasma membranes. Other aspects of infection were also affected, such as VIB formation and distribution of the outer capsid proteins. Overall, these results illustrate the intricate role of NS3 in the infection cycle. en_ZA
dc.description.department Biochemistry en_ZA
dc.description.department Genetics en_ZA
dc.description.department Microbiology and Plant Pathology en_ZA
dc.description.embargo 2020-05-01
dc.description.librarian hj2019 en_ZA
dc.description.sponsorship The University of Pretoria Institutional Research Theme, South Africa (Grant A0V004) and the Poliomyelitis Research Foundation, South Africa (Grants 13/21 and 16/26). Graduate bursary support was received from the National Research Foundation of South Africa, South Africa (Grant number 102209 and 116412), the Poliomyelitis Research Foundation, South Africa (Grant 14/91) and the University of Pretoria, South Africa. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Ferreira-Venter, L., Venter, E., Theron, J. et al. 2019, 'Targeted mutational analysis to unravel the complexity of African horse sickness virus NS3 function in mammalian cells', Virology, vol. 531, pp. 149-161. en_ZA
dc.identifier.issn 0042-6822 (print)
dc.identifier.issn 1096-0341 (online)
dc.identifier.other 10.1016/j.virol.2019.03.005
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2019 Elsevier Inc. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Virology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Virology, vol. 531, pp. 149-161, 2019. doi : 10.1016/j.virol.2019.03.005. en_ZA
dc.subject Rous sarcoma virus (RSV) en_ZA
dc.subject Pathway en_ZA
dc.subject Release en_ZA
dc.subject Gag en_ZA
dc.subject Trafficking en_ZA
dc.subject Lipid rafts en_ZA
dc.subject Insect cell en_ZA
dc.subject African horsesickness virus (AHSV) en_ZA
dc.subject Bluetongue virus (BTV) en_ZA
dc.subject Non-structural protein 3 (NS3) en_ZA
dc.subject Reverse genetics en_ZA
dc.subject Virus trafficking en_ZA
dc.subject Virus release en_ZA
dc.subject Non-structural protein 1 (NS1) en_ZA
dc.subject Protein functional domains en_ZA
dc.title Targeted mutational analysis to unravel the complexity of African horse sickness virus NS3 function in mammalian cells en_ZA
dc.type Postprint Article en_ZA

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