Identification of novel QTL for black tea quality traits and drought tolerance in tea plants (Camellia sinensis)

Show simple item record Koech, Robert K. Malebe, Pelly Masixolise Nyarukowa, Christopher Mose, Richard Kamunya, Samson M. Apostolides, Zeno 2019-05-28T09:31:41Z 2019-05-28T09:31:41Z 2018-02
dc.description The DArT sequences have been submitted to NCBI ( BioProject PRJNA398959, Supplementary Table 2. en_ZA
dc.description.abstract Tea (Camellia sinensis) contains polyphenols and caffeine which have been found to be of popular interest in tea quality. Tea production relies on well-distributed rainfall which influence tea quality. Phenotypic data for two segregating tea populations TRFK St 504 and TRFK St 524 were collected and used to identify the quantitative trait loci (QTL) influencing tea biochemical and drought stress traits based on a consensus genetic map constructed using the DArTseq platform. The populations comprised 261 F1 clonal progeny. The map consisted of 15 linkage groups which corresponds to chromosome haploid number of tea plant (2n = 2× = 30) and spanned 1260.1 cM with a mean interval of 1.1 cM between markers. A total of 16 phenotypic traits were assessed in the two populations. Both interval and multiple QTL mapping revealed a total of 47 putative QTL in the 15 LGs associated with tea quality and percent relative water content at a significant genome-wide threshold of 5%. In total, six caffeine QTL, 25 catechins QTL, three theaflavins QTL, nine QTL for tea taster score, and three QTL for percent relative water contents were detected. Out of these 47 QTL, 19 QTL were identified for ten traits in three main regions on LG01, LG02, LG04, LG12, LG13, and LG14. The QTL associated with caffeine, individual catechins, and theaflavins were clustered mostly in LG02 and LG04 but in different regions on the map. The explained variance by each QTL in the population ranged from 5.5 to 56.6%, with an average of 9.9%. Identification of QTL that are tightly linked to markers associated with black tea quality coupled with UPLC assay may greatly accelerate development of novel tea cultivars owing to its amenability at seedling stage. In addition, validated molecular markers will contribute greatly to adoption of marker-assisted selection (MAS) for drought tolerance and tea quality improvement. en_ZA
dc.description.department Biochemistry en_ZA
dc.description.librarian hj2019 en_ZA
dc.description.sponsorship Study grants for RK and PM from James Finlay (Kenya) Ltd., George Williamson (Kenya) Ltd., Sotik Tea Company (Kenya) Ltd., Mcleod Russell (Uganda) Ltd., the TRI of Kenya, and Southern African Biochemistry and Informatics for Natural Products (SABINA). The C. sinensis cultivars used in this study were provided by the TRI of Kenya. Supplementary funding was provided by the Technology and Human Resources for Industry Programme (THRIP), an initiative of the Department of Trade and Industries of South Africa (dti), the National Research Foundation (NRF) of South Africa, and the University of Pretoria (South Africa). en_ZA
dc.description.uri en_ZA
dc.identifier.citation Koech, R.K., Malebe, P.M., Nyarukowa, C. et al. Identification of novel QTL for black tea quality traits and drought tolerance in tea plants (Camellia sinensis). Tree Genetics & Genomes (2018) 14: 9. en_ZA
dc.identifier.issn 1614-2942 (print)
dc.identifier.issn 1614-2950 (online)
dc.identifier.other 10.1007/s11295-017-1219-8
dc.language.iso en en_ZA
dc.publisher Springer en_ZA
dc.rights © Springer-Verlag GmbH Germany, part of Springer Nature 2018. The original publication is available at : http://link.springer.comjournal/11295. en_ZA
dc.subject Tea (Camellia sinensis) en_ZA
dc.subject Tea quality en_ZA
dc.subject Drought tolerance en_ZA
dc.subject Ultra-performance liquid chromatography (UPLC) en_ZA
dc.subject Diversity arrays technology (DArT) en_ZA
dc.subject Linkage map en_ZA
dc.subject DArTseq en_ZA
dc.subject Quantitative trait loci (QTL) en_ZA
dc.title Identification of novel QTL for black tea quality traits and drought tolerance in tea plants (Camellia sinensis) en_ZA
dc.type Postprint Article en_ZA

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