Biological degradation of pyrene and fluoranthene in porous media and attached growth systems by biosurfactant producing bacteria

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dc.contributor.author Chirwa, Evans M.N.
dc.contributor.author Lutsinge, Tshilidzi B.
dc.date.accessioned 2019-05-15T10:04:33Z
dc.date.available 2019-05-15T10:04:33Z
dc.date.issued 2018
dc.description.abstract The dissolution and degradation of higher ring-number polycyclic aromatic compounds were tested in batch and attached growth medium using soil biopile and fixed-film bioreactor systems. Bioavailability of polycyclic aromatic hydrocarbons (PAHs) decreases with increasing ring-number. This means high ringnumber compounds will survive longer in the environment and will therefore have a longer-term impact. In the current study, pyrene and fluoranthene were degraded using a biosurfactant producing culture of Pseudomonas aeruginosa grown in fixed-bed and soil biopile experiments. High ring-number PAHs prove to be recalcitrant in nature due to their hydrophobicity and poor partitioning to soil media fixed biomass in biofilm reactors. The use of biosurfactant could facilitate faster dissolution of the PAH and thus would increase bioavailability of the PAH compounds to PAH degrading bacteria. The culture used in this study was obtained from engine oil contaminated soil, that grow on HMW PAHs and are capable of degrading PAHs were enriched, isolated and characterize. The isolated microbial cultures were further characterised 16S rDNA gene sequencing and genotype fingerprinting. During baseline study, over 90 % fluoranthene was degraded as a model compound in biofilm reactor system. Over 88 % pyrene was degraded in soil biopiles. In both cases, increased biosurfactant dose improved degradation rate up to the point where the PAH concentration became inhibitory to biomass growth and PAH degradation. en_ZA
dc.description.department Chemical Engineering en_ZA
dc.description.librarian am2019 en_ZA
dc.description.uri http://www.aidic.it/cet en_ZA
dc.identifier.citation Chirwa E.M.N., Lutsinge T.B., 2018, Biological degradation of pyrene and fluoranthene in porous media and attached growth systems by biosurfactant producing bacteria , Chemical Engineering Transactions, 70, 1777-1782, DOI: 10.3303/CET1870297. en_ZA
dc.identifier.issn 2283-9216 (online)
dc.identifier.other 10.3303/CET1870297
dc.identifier.uri http://hdl.handle.net/2263/69134
dc.language.iso en en_ZA
dc.publisher The Italian Association of Chemical Engineering en_ZA
dc.rights © 2018, AIDIC Servizi S.r.l. en_ZA
dc.subject Pseudomonas aeruginosa en_ZA
dc.subject Biosurfactant en_ZA
dc.subject Bacteria en_ZA
dc.subject Polycyclic aromatic hydrocarbon (PAH) en_ZA
dc.title Biological degradation of pyrene and fluoranthene in porous media and attached growth systems by biosurfactant producing bacteria en_ZA
dc.type Article en_ZA


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